Method for measuring oxygen concentration

• US 4,810,655 A
• Filed: 09/11/1986
• Issued: 03/07/1989
• Est. Priority Date: 07/03/1985
• Status: Expired due to Term

• Current Assignee: Hospira Incorporated (Pfizer Inc.)
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Khalil, Gamal-Eddin, Gouterman, Martin P., Green, Edmond
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): Hill, Jr., Robert J.
• Application Number:
  US06/931,746
• Time in Patent Office: 908 Days
• Field of Search: 436/136, 436/138, 436/172, 436/175, 436/178, 128/633, 128/634, 128/664, 128/665, 128/666, 250/458.1, 250/459.1, 356/39, 356/40, 356/41
• US Class Current: 436/138
• CPC Class Codes: A61K 49/0013 Luminescence
• CPC Class Codes: A61K 49/0015 Phosphorescence
• CPC Class Codes: A61K 49/0021 the fluorescent group being a small organic molecule
• CPC Class Codes: A61K 49/0036 Porphyrins used in photodynamic therapy A61K41/0071 or A61K41/0076; used as targeting group or modifying agent for targeting a therapeutic compound A61K47/546
• CPC Class Codes: G01N 2021/6432 Quenching
• CPC Class Codes: G01N 21/643 non-biological material
• CPC Class Codes: Y10T 436/207497 Molecular oxygen
• CPC Class Codes: Y10T 436/209163 Dissolved or trace oxygen or oxygen content of a sealed environment
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

Methods and compositions are described for measuring oxygen concentration, particularly for monitoring oxygen in the blood with a fiber optic catheter. Oxygen concentration is determined by observing quenching of the emission from a luminescent (phosphorescent of fluorescent) molecule embedded in an oxygen-permeable matrix. A test fluid of unknown oxygen concentration is contacted with the matrix containing at least one luminescent substance. The matrix is subjected to irradiation over some period of time by light of a wavelength that is strongly absorbed by the luminescent substance, and a measure of the time dependence of luminescent emission intensity I(t) is obtained. Three modes of determining oxygen concentration from I(t) are described.

Claims

1. A method of measuring oxygen concentration in a fluid, comprising the steps of:

(a) contacting a test fluid with a sensor composition comprising a luminescent substance, whose luminescent emission is sensitive to quenching by oxygen, admixed in an oxygen-permeable matrix, the sensor composition exhibiting a nonexponential luminescent emission when irradiated with light containing wavelengths strongly absorbed by the luminescent substance,

(b) irradiating the sensor composition with light containing wavelengths strongly absorbed by the luminescent substance,

(c) terminating the irradiation of step (b),

(d) measuring the flux of luminescent light emitted by the sensor composition during at least two time intervals comprising at least one time interval subsequent to step (c),

(e) comparing the flux values measured in step (d) to obtain a ratioing R value,

(f) determining the oxygen concentration in the test fluid by comparing the ratioing R value obtained in step (e) with a similarly obtained ratioing R value for at least one reference fluid of known oxygen concentration.

2. The method of claim 1 wherein the at least two time intervals for flux measurement in step (d) substantially encompass the period of linear decay of luminescent emission by the sensor composition subsequent to step (c).

3. The method of claim 2 wherein the at least two time intervals subdivide the period of linear decay of luminescent emission by the sensor composition subsequent to step (c) into substantially equal time intervals.

4. The method of claim 2 wherein the at least two time intervals are two in number.

5. The method of claim 4 wherein the ratioing R value is given by:

space="preserve" listing-type="equation">R=(I.sub.1 -I.sub.2)/(I.sub.1 +I.sub.2)

where I₁ is the flux of luminescent emission by the sensor composition measured during a first time interval and I₂ is the flux of luminescent emission by the sensor composition measured during a second time interval.

6. The method of claim 1 wherein the at least two time intervals for flux measurement in step (d) substantially encompass the period of detectable luminescent emission by the sensor composition subsequent to step (c).

7. The method of claim 6 wherein at least one of the at least two time intervals substantially encompasses the period of linear decay of luminescent emission by the sensor composition subsequent to step (c).

8. The method of claim 6 wherein the ratioing R value is given by:

space="preserve" listing-type="equation">R=I.sub.1 /I.sub.2

wherein I₁ is the flux of luminescent emission by the sensor composition measured during the period of linear decay of luminescent emission by the sensor composition subsequent to step (c) and I₂ is the flux of luminescent emission by the sensor composition measured during any remaining period of detectable luminescent emission by the sensor composition.

9. The method of claim 1 wherein step (b) takes place during at least one of the at least two time intervals for flux measurement.

10. The method of claim 9 wherein at least one first time interval substantially encompasses the period leading up to an emission intensity plateau by the sensor composition during step (b) and at least one second time interval substantially encompasses the period of detectable luminescent emission by the sensor composition subsequent to step (c).

11. The method of claim 10 wherein the ratioing R value is given by:

space="preserve" listing-type="equation">R=I.sub.1 /I.sub.2

wherein I₁ is the flux of luminescent emission by the sensor composition measured during the at least one first time interval and I₂ is the flux of luminescent emission by the sensor composition measured during the at least one second time interval.

17. The method of claims 1, 12, 13, 14, 15, or 16 wherein the test fluid is blood.

18. The method of claims 1, 12, or 13 wherein the test fluid is blood and the at least one reference fluid is air.

19. The method of claims 1, 12, 13, 14, 15, or 16 wherein the irradiating light and emitted light are transmitted by fiber optic means.

20. The method of claim 19 wherein the sensor composition is positioned in a light path of a single optical fiber.

21. The method of claims 1, 14, 15, or 16 wherein the luminescent substance is selected from the group consisting of phosphorescent and fluorescent substances.

22. The method of claim 21 wherein the luminescent substance is a metallo derivative of a porphyrin, chlorin, bacteriochlorin, or isobacteriochlorin.

23. The method of claim 22 wherein the luminescent substance is octaethylporphyrin, tetraphenylporphyrin, tetra(pentafluorophenyl)porphyrin, tetrabenzporphyrin, or the chlorins, bacteriochlorins, or isobacteriochlorins of said porphyrins.

24. The method of claim 22 wherein the luminescent substance is a platinum or palladium derivative of a porphyrin, chlorin, bacteriochlorin, or isobacteriochlorin.

25. The method of claim 21 wherein the luminescent substance is partially or wholly fluorine substituted.

26. The method of claims 1, 14, 15, or 16 wherein the luminescent substance comprises one or both of a platinum derivative and a palladium derivative of a tetra(pentafluorophenyl)porphyrin.

27. The method of claims 1, 12, 13, 14, 15 or 16 wherein the oxygen-permeable matrix is a plastic.

28. The method of claim 27 wherein the plastic comprises one or more of polyvinyl chloride, polymethyl metacrylate, cellulose acetate, and silicon-polybicarbonate copolymer, with or without a plasticizer.

12. A method of measuring oxygen concentration in a fluid, comprising the steps of:

(a) contacting a test fluid with a sensor composition comprising a phosphorescent substance, whose phosphorescent emission is sensitive to quenching by oxygen, admixed in an oxygen-permeable matrix, the sensor composition exhibiting a nonexponential phosphorescent emission when irradiated with light containing wavelengths strongly absorbed by the luminescent substance,

(b) irradiating the sensor composition with light containing wavelengths strongly absorbed by the phosphorescent substance,

(c) terminating the irradiation of step (b),

(d) measuring the intensity of phosphorescent light emitted by the sensor composition at a plurality of times subsequent to step (c),

(e) fitting the measured intensity values as follows;

space="preserve" listing-type="equation">I(t)=A.sub.1 e.sup.-k.sbsp.1.sup.t +A.sub.2 e.sup.-k.sbsp.2.sup.t

wherein I(t) is the measured intensity at time t, e is the exponential function, and A₁, k₁, A₂, and k₂ are fitting parameters,

(f) determining the average decay rate of phosphorescent emission by the sensor composition, k, from the fitting parameters in step (e) as follows;

space="preserve" listing-type="equation">k=(A.sub.1 k.sub.1 +A.sub.2 k.sub.2)/(A.sub.1 +A.sub.2),

(g) determining the oxygen concentration in the test fluid by comparing the average decay rate determined in step (f) with a similarly obtained k value for at least one reference fluid of known oxygen concentration.

29. The method of claim 12 or 13 wherein the phosphorescent substance is a metallo derivative of a porphyrin, chlorin, bacteriochlorin, or isobacteriochlorin.

30. The method of claim 29 wherein the phosphorescent substance is octaethylporphyrin, tetraphenylporphyrin, tetra(pentafluorophenyl)porphyrin, tetrabenzporphyrin, or the chlorins, bacteriochlorins, or isobacteriochlorins of said porphyrins.

31. The method of claim 29 wherein the phosphorescent substance is a platinum or palladium derivative of a porphyrin, chlorin, bacteriochlorin, or isobacteriochlorin.

32. The method of claim 29 wherein the phosphorescent substance is partially or wholly fluorine substituted.

33. The method of claim 12 or 13 wherein the phosphorescent substance comprises one or both of a platinum derivative and a palladium derivative of a tetra(pentafluorophenyl)porphyrin.

13. A method of measuring oxygen concentration in a fluid, comprising the steps of:

(a) contacting a test fluid with a sensor composition comprising a phosphorescent substance, whose phosphorescent emission is sensitive to quenching by oxygen, admixed in an oxygen-permeable matrix, the sensor composition exhibiting a nonexponential phosphorescent emission when irradiated with light containing wavelengths strongly absorbed by the phosphorescent substance,

(b) irradiating the sensor composition with light containing wavelengths strongly absorbed by the phosphorescent substance,

(c) terminating the irradiation of step (b),

(d) measuring the intensity of phosphorescent light emitted by the sensor composition at a plurality of times subsequent to step (c),

(e) fitting the measured intensity values as follows;

space="preserve" listing-type="equation">I(t)=A.sub.1 e.sup.-k.sbsp.1.sup.t +A.sub.2 e.sup.-k.sbsp.2.sup.t

wherein I(t) is the measured intensity at time t, e is the exponential function, and A₁, k₁, A₂, and k₂ are fitting parameters,

(f) determining the average decay time of phosphorescent emission by the sensor composition, τ

, from the fitting parameters in step (e) as follows;

space="preserve" listing-type="equation">τ

=(A.sub.1 k.sub.1.sup.-1 +A.sub.2 k.sub.2.sup.-1)/(A.sub.1 +A.sub.2),

(g) determining the oxygen concentration in the test fluid by comparing the average decay time determined in step (f) with a similarly obtained τ

value for at least one reference fluid of known oxygen concentration.

14. A method of measuring oxygen concentration in a fluid, comprising the steps of:

(a) contacting a test fluid with a sensor composition comprising a luminescent substance, whose luminescent emission is sensitive to quenching by oxygen, admixed in an oxygen-permeable matrix, the sensor composition exhibiting a nonexponential luminescent emission when irradiated with light containing wavelengths strongly absorbed by the luminescent substance,

(b) irradiating the sensor composition with light containing wavelengths strongly absorbed by the luminescent substance,

(c) terminating the irradiation of step (b),

(d) measuring the intensity of luminescent light emitted by the sensor composition at a plurality of times subsequent to step (c),

(e) from the measured intensities in step (d), determining the slope of emission decay during the period of linear decay of luminescent emission by the sensor composition,

(f) determining the oxygen concentration in the test fluid by comparing the slope value obtained in step (e) with a similarly obtained slope value for at least one reference fluid of known oxygen concentration.

15. A method of measuring oxygen concentration in a fluid, comprising the steps of:

(a) contacting a plurality of reference fluids of known oxygen concentration with a sensor composition comprising a luminescent substance, whose luminescent emission is sensitive to quenching by oxygen, admixed in an oxygen-permeable matrix, the sensor composition exhibiting a nonexponential luminescent emission when irradiated with light containing wavelengths strongly absorbed by the luminescent substance,

(b) irradiating the sensor composition with light containing wavelengths strongly absorbed by the luminescent substance,

(c) terminating the irradiation of step (b),

(d) measuring the intensity of luminescent light emitted by the sensor composition during a plurality of times subsequent to step c),

(e) from the measured intensities in step (d), determining the intensity versus time profile of emission decay for each reference fluid during the period of linear decay of luminescent emission by the sensor composition,

(f) contacting a test fluid of unknown oxygen concentration with the sensor composition and repeating steps (b) and (c) with the test fluid,

(g) measuring the time it takes for the intensity of luminescent light emitted by the sensor composition subsequent to step (f) to fall to a predetermined intensity value which can be referenced against the intensity versus time profiles determined in step (e), and

(h) determining the oxygen concentration in the test fluid by comparing the time value measured in step (g) with the intensity versus time profiles determined in step (e) for the reference fluids of known oxygen concentrations.

16. A method of measuring oxygen concentration in a fluid, comprising the steps of:

(a) contacting a plurality of reference fluids of known oxygen concentration with a sensor composition comprising a luminescent substance, whose luminescent emission is sensitive to quenching by oxygen, admixed in an oxygen-permeable matrix, the sensor composition exhibiting a nonexponential luminescent emission when irradiated with light containing wavelengths strongly absorbed by the luminescent substance,

(b) irradiating the sensor composition with light containing wavelengths strongly absorbed by the luminescent substance,

(c) terminating the irradiation of step (b),

(d) measuring the intensity of luminescent light emitted by the sensor composition during a plurality of times subsequent to step (c),

(e) from the measured intensities in step (d), determining the intensity versus time profile of emission decay of each reference fluid during the period of linear decay of luminescent emission by the sensor composition,

(f) contacting a test fluid of unknown oxygen concentration with the sensor composition and repeating steps (b) and (c) with the test fluid,

(g) measuring the intensity of luminescent light emitted by the sensor composition at a given time interval subsequent to step (f), which time interval falls within the period of linear decay for the sensor composition, and

(h) determining the oxygen concentration in the test fluid by comparing the intensity value measured in step (g) with the intensity versus time profiles determined in step (e) for the reference fluids of known oxygen concentrations.