Enzyme electrode and membrane

• US 4,832,797 A
• Filed: 11/25/1986
• Issued: 05/23/1989
• Est. Priority Date: 11/28/1985
• Status: Expired due to Term

• Current Assignee: Hypoguard USA Inc.
• Original Assignee: Imperial Chemical Industries PLC (IMPERIAL CHEMICAL INDUSTRIES)
• Inventors: Mullen, William H., Vadgama, Pankaj M., Scott, Graham W.
• Primary Examiner(s): Niebling, John F.
• Assistant Examiner(s): MARQUIS, STEVEN
• Application Number:
  US06/936,720
• Time in Patent Office: 910 Days
• Field of Search: 204/403, 204/415, 204/1 T, 210/500.27, 210/500.41
• US Class Current: 205/778
• CPC Class Codes: C12N 11/089 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: C12Q 1/002 Electrode membranes

Abstract

A membrane particularly for use in a sensor of the enzyme-electrode type which comprises one or more layers of material and an enzyme-containing layer and in which one layer is formed from a sulphonated or unsulphonated polyarylsulphone or a sulphonated or unsulphonated polyarylketone. The specification also relates to a non-enzymic sensor having a membrane including a layer formed from one of these polymers. Methods for determining an analyte using an enzymic or non-enzymic sensors are also included in the scope of the invention.

Claims

1. An enzyme electrode sensor which comprises an electrode and a membrane permeable to liquids and solutes said membrane comprising an enzyme-containing layer and at least one layer of material positioned between the enzyme containing layer and the electrode wherein said at least one layer of material is formed from a homogeneous polymer selected from the group consisting of a sulphonated and an unsulphonated polyarylsulphone and a sulphonated and an unsulphonated polyarylketone.

2. A sensor according to claim 1 in which the membrane comprises a first layer of material positioned between the enzyme-containing layer and the electrode, the enzyme-containing layer and a second layer of material on the other side of the enzyme-containing layer wherein the first layer is formed from a polymer selected from the group consisting of a sulphonated and an unsulphonated polyarylsulphone and a sulphonated and an unsulphonated polyarylketone.

3. A sensor according to claim 1 wherein the first layer has a thickness in the range of 0.2 to 1.0 microns.

4. A sensor according to claim 1 wherein the second layer is formed from a polymeric material having a percentage porosity in the range 0.001% to 0.5%.

5. A method for determining an analyte in a specimen which comprises bringing the specimen into contact with the outer face of a membrane, permeable to liquids and solutes and comprising an enzyme, in the presence of which the analyte is convertable into a species detectable by a sensor which comprises an electrode and the membrane, and one or more layers of material, and measuring the response of the sensor to the species, wherein at least one layer of material is formed from a homogeneous polymer selected from the group consisting of a sulphonated and an unsulphonated polyarylsulphone and a sulphonated and an unsulphonated polyarylketone.