Electrofusion cell and method of making the same

• US 4,832,814 A
• Filed: 12/28/1987
• Issued: 05/23/1989
• Est. Priority Date: 12/28/1987
• Status: Expired due to Term

• Current Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Original Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Inventors: Root, Richard T.
• Primary Examiner(s): Niebling, John F.
• Assistant Examiner(s): STARSIAK, JOHN S
• Application Number:
  US07/138,040
• Time in Patent Office: 512 Days
• Field of Search: 435/173, 435/172.2, 435/172.1, 435/287, 435/289, 935/89, 935/91, 935/93, 935/94, 204/299 R, 204/180.1, 156/634, 156/645, 156/652, 156/656, 156/659.1, 430/320, 430/312, 430/313, 430/318, 430/325, 430/394
• US Class Current: 435/285.2
• CPC Class Codes: C12M 35/02 Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Abstract

An apparatus and method of use is provided for the fusion of different cell types into hybrid cells through the use of electrofusion. The apparatus consists of thin-film electrodes etched onto an optically clear lower plate, a channel defined at its boundaries by a photocopolymer material laminated between the lower plate and an upper optically clear top plate providing closure of the channel as well as entry for the cells through the use of integral Luer ports. The apparatus may be produced, with minor adjustment, by using phototools.

Claims

1. An apparatus for fusing cells comprising:

a laminate formed by a first planar member

a grid of opposed interdigitated electrodes coated on the first member,

a second planar member,

a photopolymer, that is resistant to water, having a high dielectric, sandwiched between the first and second members, the photopolymer and members defining a cavity encompassing at least a portion of the opposed electrodes, means for applying electrical signals to the electrodes, and

means in the second member defining a pair of ports for admitting liquids and cells to the chamber.

2. The apparatus of claim 1 wherein the photopolymer is sheetlike, has low leachability to chemicals toxic to living cells, and has a thickness in range of about 0.01 to about 0.02 cm.

3. The apparatus of claim 2 wherein the photopolymer has a thickness of about 0.015 cm.

4. The apparatus of claim 2 wherein the photopolymer is comprised of two sheetlike layers, each defining the cavity.

5. The apparatus of claim 4 wherein the one layer adjacent the first member is developed.

6. The apparatus of claim 5 wherein the other layer adjacent the second member is undeveloped and secures the one layer and second member together.

7. The apparatus of claim 5 where the other layer adjacent the second member is developed.

8. The apparatus of claim 1 wherein the photopolymer is comprised of two sheetlike layers, each defining the cavity.

9. The apparatus of claim 8 wherein the one layer adjacent the first member is developed.

10. The apparatus of claim 9 wherein the other layer adjacent the second member is undeveloped and secures the one layer and second member together.

11. The apparatus of claim 1 wherein the electrodes are chromium.

12. The apparatus of claim 1 wherein the electrodes are a mixture of the oxides of indium and tin.

13. The apparatus of claim 1 wherein the first member is glass.

14. The apparatus of claim 2 wherein the first member is glass.

15. The apparatus of claim 1 wherein the second member is polycarbonate.

16. The apparatus of claim 15 wherein the first member is glass.