Sensor system

• US 4,833,091 A
• Filed: 02/06/1987
• Issued: 05/23/1989
• Est. Priority Date: 02/06/1987
• Status: Expired due to Fees

• Current Assignee: Medtronic Minimed Incorporated (Medtronic Plc)
• Original Assignee: Shiley Incorporated
• Inventors: Leader, Matthew J., Kamiya, Tadao
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): MCMAHON, TIMOTHY M
• Application Number:
  US07/012,105
• Time in Patent Office: 837 Days
• Field of Search: 436/68, 436/163, 436/172, 436/178, 436/133, 422/52, 422/68, 128/632, 128/635
• US Class Current: 436/133
• CPC Class Codes: G01N 2021/0385 Diffusing membrane; Semipermeable membrane
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 2021/6434 Optrodes
• CPC Class Codes: G01N 2021/6441 with two or more labels
• CPC Class Codes: G01N 2021/772 Tip coated light guide
• CPC Class Codes: G01N 2021/775 Indicator and selective membrane
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/643 non-biological material
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G01N 21/80 Indicating pH value
• CPC Class Codes: Y10T 436/204998 Inorganic carbon compounds
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

A method of measuring the concentration of carbon dioxide in a medium by determining the water content in a pH-independent sensor system comprising an optical fiber having attached thereto a fluorescence indicator embedded in a carrier matrix containing a miscible mixture of water and non-aqueous solvent in predetermined proportions and being separated from said medium by a gas-permeable, water-impermeable diffusion membrane, said indicator, when excited by excitation radiation of a predetermined wavelength, emitting fluorescent emission whose intensity is dependent upon the ratio of water to non-aqueous solvent present in the system such that the ratio of intensities of emitted radiation is therefore proportional to the amount of water present and diffusion of carbon dioxide through said gas-permeable membrane and subsequent reaction with water to deplete the water content of the system induces a change in the intensities of said emissions, which method comprises transmitting excitation radiation through said optical fiber and measuring the ratio of the intensities of the emitted radiation, thereby obtaining a determination of the water content and calculating therefrom the carbon dioxide concentration in the surrounding medium.

Claims

1. A method of measuring the concentration of carbon dioxide in a medium by determining the water content in a pH-independent sensor system comprising an optical fiber having a proximal end and a distal end, said distal end having attached thereto a fluorescence indicator embedded in a carrier matrix with a predetermined water content, said carrier matrix containing a miscible mixture of water and non-aqueous solvent in predetermined proportions and being separated from said medium by a gas-permeable, water-impermeable diffusion membrane, said indicator, when excited by excitation radiation of a predetermined wavelength λ

o, emitting fluorescent emission at a wavelength λ

_w in the presence of water and at a wavelength λ

_s in the presence of said non-aqueous solvent, the intensity of each emission being independent upon the ratio of water to non-aqueous solvent present in the system such that the ratio of the intensities of emitted radiation of wavelengths λ

_w and λ

_s is therefore proportional to the amount of water present and diffusion of carbon dioxide through said gas-permeable membrane and subsequent reaction with water to deplete the water content of the system induces a change in the intensities of said emissions, which method comprises transmitting through said optical fiber, from a source adjacent to its proximal end, excitation radiation of said predetermined wavelength λ

_o, and measuring the ratio of the intensities of the emitted radiation of wavelengths λ

_w and λ

_s, thereby obtaining a determination of the water content and calculating therefrom the carbon dioxide concentration in the surrounding medium.

2. A method according to claim 1, in which said gas-permeable membrane is a silicone rubber membrane.

3. A method according to claim 1, in which said fluorescence indicator is 8-hydroxy-1,3,6-pyrenetrisulfonic acid (HPTA) which emits a fluorescent emission at a peak wavelength λ

_w of 510 nm in the presence of water and a fluorescent emission at a peak wavelength λ

_s of 440 to 460 nm in the presence of non-aqueous solvent when excited with laser radiation of a wavelength λ

o of 337 nm.

4. A method according to claim 3, in which said non-aqueous solvent is ethylene glycol or ethanol.