Method and reagent for determination of an analyte via enzymatic means using a ferricyanide/ferric compound system
First Claim
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1. Composition useful in determining an analyte in a sample, comprising:
- (a) an enzyme which specifically reacts with said analyte;
(b) a soluble ferricyanide compound which is reducible in the presence of an electron to produce a ferrocyanide compound;
(c) a soluble ferric compound which reacts with a ferrocyanide compound to form a reaction product therebetween, and(d) a buffer which does not prevent formation of said reaction product, wherein said composition has a pH of from about 3.0 to about 6.0.
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Abstract
A composition useful in determining analytes, such as glucose or cholesterol, is disclosed. The composition contains an enzyme which specifically reacts with the analyte to be determined, a soluble ferricyanide compound which is reducible to form a ferrocyanide, a soluble ferric compound which provides ferric ions for reacting with ferrocyanide to form a reaction product, and a buffer which does not prevent formation of this reaction product. The composition has a pH of from about 3.0 to about 6.0.
45 Citations
23 Claims
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1. Composition useful in determining an analyte in a sample, comprising:
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(a) an enzyme which specifically reacts with said analyte; (b) a soluble ferricyanide compound which is reducible in the presence of an electron to produce a ferrocyanide compound; (c) a soluble ferric compound which reacts with a ferrocyanide compound to form a reaction product therebetween, and (d) a buffer which does not prevent formation of said reaction product, wherein said composition has a pH of from about 3.0 to about 6.0. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. Analytical element useful in determining an analyte in a sample, comprising:
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(a) a support carrier; (b) a reagent layer applied to said support carrier, said reagent layer comprising (i) an inert film, (ii) an enzyme which specifically reacts with said analyte, (iii) a soluble ferricyanide compound which is reduced in the presence of an electron to a ferrocyanide compound, (iv) a soluble ferric compound which reacts with said ferrocyanide compound to form a reaction product, and (v) a buffer which does not prevent formation of said reaction product, wherein said reagent layer has a pH of from about 3.0 to about 6.0. - View Dependent Claims (20, 21, 22, 23)
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Specification