Detectable molecules, method of preparation and use
First Claim
1. A detectable molecule of the formula A3 --(X--R1 --E--Detb)m where A3 is A2, an oligo-or polysaccharide, an olgo- or polypeptide or a synthetic polymer, each of said oligo- or polysaccharide, oligo- or polypeptide or synthetic polymer having at least one modifiable reactive group consisting of amino, hydroxy, 1,2-cis diOH, halide aryl, imidazoyl, carbonyl, carboxyl, thiol or a residue comprising an activated carbon;
- A2 is a specific binding ligand other than an oligo- or polysaccharide, oligo- or polypeptide or an oligo- or polymucleotide and has a molecular weight of less than about 2,000;
--X-- is selected from the group consisting of ##STR52## --N═
N--, --NHSO2 --, --OSO2 --, --NH--N═
N--, ##STR53## -CH2 --NH, ##STR54## --NH--CH2 --, ##STR55## --S--CH2 --;
--R1 -- is selected from the group consisting of ##STR56## and a C1 -C10 branched or unbranched alkyl or aralkyl, which may be substituted by --OH;
--Y-- is a direct bond to --E--, or --Y-- is --E--R2 -- where -R2 -- is a C1 -C10 branched or unbranched alkyl;
Za is chlorine, bromine or iodine;
--E-- is O, NH or an acyclic divalent sulfur atom;
Detb is a chemical moiety capable of being detected, comprising biotin or a substituted or unsubstituted metal chelator or a compound capable of yielding a metal chelator; and
m is an integer from 1 to the total number of modified reactive groups on A3.
1 Assignment
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Accused Products
Abstract
A detectable molecule of the formula
A.sup.3 --(--X--R.sup.1 --E--Det.sup.b).sub.m
where A3 is A2 or a polymer, where A3 has at least one modifiable reactive group selected from the group consisting of amino, hydroxy, cis OH, halides, aryl, imidazoyl, carbonyl, carboxy, thiol or a residue comprising an activated carbon; --X-- is selected from the group consisting of ##STR1## a C1 -C10 branched or unbranched alkyl or aralkyl, which may be substituted by --OH; --Y-- is a direct bond to --E--, or --Y-- is --E--R2 -- where R is a C1 -C10 branched or unbranched alkyl; Za is chlorine, bromine or iodine; E is O, NH or an acyclic divalent sulfur atom; Detb is a chemical moiety capable of being detected, preferably comprising biotin or a metal chelator of the formula: ##STR2## or the 4-hydroxy or acyloxy derivative thereof, where R3 is C1 -C4 alkyl or CH2 COOM, M is the same or different and selected from the group consisting of hydrogen, a metal or non-metal cation or is C1 -C10 alkyl, aryl or aralkyl; and m is an integer from 1 to the total number of modified reactive groups on A3. The detectable molecules are useful in in vitro or in vivo assays or therapy.
111 Citations
42 Claims
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1. A detectable molecule of the formula A3 --(X--R1 --E--Detb)m where A3 is A2, an oligo-or polysaccharide, an olgo- or polypeptide or a synthetic polymer, each of said oligo- or polysaccharide, oligo- or polypeptide or synthetic polymer having at least one modifiable reactive group consisting of amino, hydroxy, 1,2-cis diOH, halide aryl, imidazoyl, carbonyl, carboxyl, thiol or a residue comprising an activated carbon;
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A2 is a specific binding ligand other than an oligo- or polysaccharide, oligo- or polypeptide or an oligo- or polymucleotide and has a molecular weight of less than about 2,000; --X-- is selected from the group consisting of ##STR52## --N═
N--, --NHSO2 --, --OSO2 --, --NH--N═
N--, ##STR53## -CH2 --NH, ##STR54## --NH--CH2 --, ##STR55## --S--CH2 --;
--R1 -- is selected from the group consisting of ##STR56## and a C1 -C10 branched or unbranched alkyl or aralkyl, which may be substituted by --OH;--Y-- is a direct bond to --E--, or --Y-- is --E--R2 -- where -R2 -- is a C1 -C10 branched or unbranched alkyl; Za is chlorine, bromine or iodine; --E-- is O, NH or an acyclic divalent sulfur atom; Detb is a chemical moiety capable of being detected, comprising biotin or a substituted or unsubstituted metal chelator or a compound capable of yielding a metal chelator; and m is an integer from 1 to the total number of modified reactive groups on A3. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42)
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25. The molecule of claim 111 wherein R1 is --CH2 --CH2 --.
Specification
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- Resources
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Current AssigneeEnzo Diagnostics, Inc.
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Original AssigneeEnzo Biochem Incorporated
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InventorsStavrianopoulos, Jannis G.
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Primary Examiner(s)Nucker, Christine M.
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Application NumberUS07/043,670Time in Patent Office1,183 DaysField of Search435/7, 436/537, 436/804, 534/11-14, 530/390, 536/17.1US Class Current435/7.5CPC Class CodesA61K 51/0497 conjugates with a carrier b...A61K 51/1093 conjugates with carriers be...G01N 33/533 with fluorescent labelG01N 33/553 Metal or metal coatedY10S 436/804 Radioisotope, e.g. radioimm...
