Human stem cells and monoclonal antibodies

• US 4,965,204 A
• Filed: 06/01/1987
• Issued: 10/23/1990
• Est. Priority Date: 02/06/1984
• Status: Expired due to Term

• Current Assignee: Johns Hopkins University
• Original Assignee: Johns Hopkins University
• Inventors: Civin, Curt I.
• Primary Examiner(s): Moskowitz, Margaret
• Assistant Examiner(s): Cunningham, Thomas M.
• Application Number:
  US07/055,942
• Time in Patent Office: 1,240 Days
• Field of Search: 424/88, 424/89, 530/387, 435/240.27
• US Class Current: 530/388.7
• CPC Class Codes: C07K 16/28 against receptors, cell surface antigens or cell surface determinants
• CPC Class Codes: Y10S 530/809 Fused cells, e.g. hybridoma

Abstract

Monoclonal antibodies that recognize a stage-specific antigen on immature human marrow cells are provided. These antibodies are useful in methods of isolating cell suspensions from human blood and marrow that can be employed in bone marrow transplantation. Cell suspensions containing human pluripotent lympho-hematopoietic stem cells are also provided, as well as therapeutic methods employing the cell suspensions.

Claims

1. A monoclonal antibody which specifically binds to an antigen on non-malignant, immature human marrow cells, wherein said antigen is stage specific and not lineage dependent, and said antigen is also specifically bound by the antibody produced by the hybridoma deposited under ATCC Accession No. HB-8483;

(a) which antigen is present on non-malignant, human blood or bone marrow;

(i) colony-forming cells for granulocytes and monocytes (CFC-GM),

(ii) colony-forming cells for erythrocytes (BFU-E),

(iii) colony-forming cells for eosinophils (CFC-Eo),

(iv) multipotent colony-forming cells (CFC-GEMM), and

(v) immature lymphoid precursor cells;

(b) which antigen is present on a maximum of about 5% non-malignant, human marrow cells and a maximum of about 1% non-malignant, human peripheral blood cells; and

(c) which antigen is not present on non-malignant, mature human myeloid and lymphoid cells.

2. The monoclonal antibody of claim 1 that corresponds to the monoclonal antibody produced by the hybridoma deposited under ATCC Accession No. HB-8483.

3. The monoclonal antibody of claim 1 that is the antibody produced by the hybridoma deposited under ATCC Accession No. HB-8483.

4. A hydbridoma which produces a monoclonal antibody of claim 1.

5. A hybridoma which produces a monoclonal antibody of claim 2.

6. The hybridoma deposited under ATCC Accession No. HB-8483.