Method and apparatus for electrochemical analysis

• US 4,966,671 A
• Filed: 06/20/1988
• Issued: 10/30/1990
• Est. Priority Date: 10/31/1985
• Status: Expired due to Term

• Current Assignee: Inverness Medical Switzerland GmbH (Abbott Laboratories Incorporated)
• Original Assignee: Unilever Patent Holdings BV (Unilever PLC)
• Inventors: Nylander, Claes I., Burns, Ian W.
• Primary Examiner(s): Niebling, John F.
• Assistant Examiner(s): Hsing, Ben C.
• Application Number:
  US07/208,806
• Time in Patent Office: 862 Days
• Field of Search: 204/1 T, 204/400, 204/1 A, 204/403, 204/415, 204/416, 204/417, 204/418, 204/1 N
• US Class Current: 205/780.5
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

A potentiometric electrochemical analysis method, which comprises placing by capillary action an aqueous sample liquid to be tested for the presence of a quantity of an analyte in the sample liquid, in each of two compartments of an electrochemical analysis cell, wherein the aqueous sample liquid so placed in each compartment is in electrochemical contact with one of two electrodes forming part of the cell, wherein the electrodes are insensitive to the analyte, and wherein the compartments are separated by an electroactive barrier which is sensitive to or selective for the analyte, the barrier not being in electrical contact with either electrode except through the medium of the sample liquid, the method further comprising changing the amount of the analyte material in a selected one of the compartments by adding thereto a predefined standard quantity of analyte, measuring the resulting cell potential and determining the concentration of the analyte in the sample liquid, the predefined standard quantity of analyte being allowed to dissolve in the sample liquid from a dry material predosed in one of the compartments, the dry material being free from any electroactive material to which the electrode are sensitive.

Claims

1. A potentiometric electrochemical analysis method, which comprises placing by capillary action an aqueous sample liquid to be tested for the presence of a quantity of an analyte in said sample liquid, in each of two compartments of an electrochemical analysis cell, wherein the aqueous sample liquid so placed in each compartment is in electrochemical contact with one of two electrodes forming part of the cell, wherein the electrodes are insensitive to the analyte, and wherein the compartments are separated by an electroactive barrier which is sensitive to or selective for the analyte, said barrier not being in electrical contact with either electrode except through the medium of the sample liquid, said method further comprising changing the amount of the analyte material in a selected one of the compartments by adding thereto a predefined standard quantity of analyte, measuring the resulting cell potential and determining therefrom the concentration of analyte in the sample liquid being tested, the predefined standard quantity of analyte being allowed to dissolve in said sample liquid from a dry material being free from any electroactive material to which the electrodes are sensitive.

2. A method according to claim 1, wherein said analyte comprises potassium, sodium or ammonium ions, said electrodes are halide-sensitive electrodes, said dry material is a halide-free salt of the analyte ion, and said electroactive barrier is plasticised pvc membrane containing an effective quantity of a corresponding ionophore to confer electrochemical sensitivity or selectivity for said ion thereon.