Method and structures employing chemically-labelled polynucleotide probes

  • US 4,994,373 A
  • Filed: 07/20/1989
  • Issued: 02/19/1991
  • Est. Priority Date: 01/27/1983
  • Status: Expired due to Term
First Claim
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1. A method for detecting a polynucleotide sequence which comprises:

  • fixing said polynucleotide sequence to a solid support which comprises or is contained within a transparent or translucent, non-porous system, such that a single-strand of the polynucleotide is capable of hybridizing to complementary nucleic acid sequences;

    forming an entity comprising said polynucleotide sequence hybridized to a polynucleotide or oligonucleotide probe, said probe having attached thereto a chemical label further comprising a signalling moiety capable of generating a soluble signal; and

    generating and detecting said soluble signal.

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