Fiber-optic physiological probes
First Claim
1. A method of manufacturing a fiber-optic sensor suitable for monitoring physiological analyte concentration, comprising the steps of:
- casting a polymer film of substantially uniform thickness, the polymer film being permeable to an analyte in solution and comprising a covalently-linked or admixed indicator molecule capable of responding to the analyte in an optically detectable manner;
cutting the film to produce a disc-shaped indicator matrix of substantially uniform thickness; and
,affixing the indicator matrix to one end of an optical fiber segment.
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Accused Products
Abstract
Methods of monitoring analyte concentration in fluids using fiber optic sensors that include analyte permeable matrices disposed in a light path at one end of an optical fiber waveguide. The analyte permeable matrix includes an indicator molecular capable of responding to the analyte in an optically detectable manner. The absorbance or emission from the analyte permeable matrix after irradiation is used to determine the concentration of the analyte in the fluid as a function of the measured absorbance or emission. Methods for manufacturing fiber optic sensors useful in a method of monitoring analyte concentration in a fluid include casting a polymer film that includes an indicator molecule capable of responding to the analyte in an optically detectable manner. Disks or pellets are punched from the film and applied to the end of an optical fiber waveguide.
90 Citations
11 Claims
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1. A method of manufacturing a fiber-optic sensor suitable for monitoring physiological analyte concentration, comprising the steps of:
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casting a polymer film of substantially uniform thickness, the polymer film being permeable to an analyte in solution and comprising a covalently-linked or admixed indicator molecule capable of responding to the analyte in an optically detectable manner; cutting the film to produce a disc-shaped indicator matrix of substantially uniform thickness; and
,affixing the indicator matrix to one end of an optical fiber segment. - View Dependent Claims (2, 3, 4)
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5. A method of monitoring analyte concentration in a fluid, comprising the steps of:
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contacting a fluid with a fiber-optic sensor comprising an analyte-permeable matrix disposed in a light path defined by an axial core at one end of an optical fiber segment, the analyte-permeable matrix comprising an indicator molecule covalently linked to a polymer, the indicator molecule capable of responding to the analyte in an optically detectable manner, and the polymer selected from the group consisting of methyl methacrylate/methacrylamidopropyltrimethylammonium chloride, N-vinylpyrrolidone/p-aminostyrene, methyl methacrylate/hydroxymethyl methacrylate, methyl methacrylate/N-vinylpyrrolidone, and methyl methacrylate/acrylic acid; irradiating the analyte-permeable matrix through the optical fiber segment at a first wavelength band corresponding to a region of analyte-dependent absorbance by the indicator molecule; measuring absorbance by or emission from the analyte-permeable matrix at a predetermined second wavelength band; and determining the concentration of the analyte in the fluid as a function of the measured absorbance or emission. - View Dependent Claims (6, 7)
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8. A method of manufacturing a fiber-optic sensor suitable for monitoring physiological analyte concentration, comprising the steps of:
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casting a polymer film of substantially uniform thickness, the polymer film being permeable to an analyte in solution and comprising a covalently linked or admixed indicator molecule capable of responding to the analyte in an optically detectable manner; punching the film to produce a disc-shaped indicator matrix of substantially uniform thickness; and affixing the indicator matrix to one end of an optical fiber segment. - View Dependent Claims (9, 10, 11)
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Specification