Method for monitoring glucose

• US 5,001,054 A
• Filed: 07/31/1990
• Issued: 03/19/1991
• Est. Priority Date: 06/26/1986
• Status: Expired due to Fees

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Wagner, Daniel B.
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): McMahon, Timothy M.
• Application Number:
  US07/560,913
• Time in Patent Office: 231 Days
• Field of Search: 422/58, 422/82.06, 422/82.07, 422/82.08, 356/39, 356/445, 128/637, 128/665, 435/14, 435/25, 435/288, 435/310, 436/95, 436/136, 436/138, 436/800, 436/805, 436/904, 424/9
• US Class Current: 435/14
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1459 invasive, e.g. introduced into the body by a catheter A61B5/1464 takes precedence
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/904 Oxidation - reduction indicators
• CPC Class Codes: Y10T 436/10 Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
• CPC Class Codes: Y10T 436/144444 Glucose
• CPC Class Codes: Y10T 436/207497 Molecular oxygen
• CPC Class Codes: Y10T 436/209163 Dissolved or trace oxygen or oxygen content of a sealed environment

Abstract

A method for monitoring the glucose level in a body fluid uses an apparatus which includes a conjugate of glucose oxidase and a fluorescent dye coated onto an optical fiber in contact with the body fluid, a source of excitation light and a fluorescence emission detector. Glucose is oxidized by oxygen in the body fluid causing a decrease in oxygen concentration at the enzyme. The fluorescent dye is sensitive to oxygen quenching so that, when the oxygen concentration decreases, fluorescence emission increases in direct proportion to the glucose concentration in the fluid.

Claims

1. A method for detecting an elevated glucose level in a body fluid comprising:

(a) contacting a body fluid containing glucose with a first solid support having immobilized thereon a first conjugate comprising active glucose oxidase and a fluorescent dye, fluorescence emission from said dye being sensitive to oxygen quenching, and with a second solid support having immobilized thereon a second conjugate comprising inactive glucose oxidase and the same aforementioned fluorescent dye whereby said glucose is oxidized by oxygen in said fluid at said first conjugate but not at said second conjugate;

(b) applying excitation light to said first and second conjugates;

(c) detecting fluorescence emission from said first and second conjugates;

(d) comparing the intensities of emission from said first and second conjugates; and

(e) determining that an elevated glucose level exists in said fluid if said intensity of emission from said first conjugate is greater than said intensity of emission from said second conjugate.

2. The method of claim 1 wherein said body fluid is contacted in vivo in a blood stream of a living being.

3. The method of claim 1 wherein said body fluid is contacted in vitro, said body fluid being selected from the group of fluids consisting of blood and urine.

4. The method of claim 1 wherein said fluorescent dye is selected from the group of dyes consisting of perylen dibutyrate and fluoranthrene.

5. The method of claim 1 wherein said dye is covalently conjugated to said active and said inactive glucose oxidase.

6. The method of claim 4 further comprising a linking group between said dye and said glucose oxidase.

7. The method of claim 1 wherein said first and second solid supports are optical fibers.

8. A method for detecting an elevated glucose level in a body fluid comprising:

(a) contacting a body fluid containing glucose with first, second, third and fourth optical fibers, said first and second fibers having immobilized thereon a first covalent conjugate of glucose oxidase and a fluorescent dye, fluorescence emission from said dye being subject to oxygen quenching, said first conjugate being coated with a membrane selectively permeable to molecules the size of glucose and smaller, said third and fourth optical fibers having immobilized thereon a second covalent conjugate of glucose oxidase and the same aforementioned fluorescent dye, said second conjugate being coated with a membrane selectively permeable to oxygen but impermeable to glucose, whereby said glucose is oxidized by oxygen in said fluid at said first conjugate ,but not at said second conjugate;

(b) applying excitation light to said first conjugate through said first fiber and to said second conjugate through said third fiber;

(c) detecting fluorescence emission from said first conjugate through said second fiber and from said second conjugate through said fourth fiber;

(d) comparing the intensity of emission from said first conjugate with the intensity of emission from said second conjugate; and

(e) determining that an elevated glucose level exists in said fluid if said intensity of emission from said first conjugate is greater than said intensity of emission from said second conjugate.

9. A method for quantitating an elevated concentration of glucose in a body fluid comprising:

(a) contacting a body fluid containing glucose with a covalent conjugate comprising immobilized active glucose oxidase and a fluorescent dye, fluorescence emission from said dye being sensitive to oxygen quenching, whereby glucose is oxidized by oxygen in said fluid at said conjugate;

(b) applying excitation light to said conjugate;

(c) measuring fluorescence emission from said conjugate;

(d) quantitating an elevated concentration of glucose in a body fluid by comparing the magnitude of said emission with the magnitude of fluorescence emission measured when a fluid containing a known quantity of glucose is subjected to steps (a) to (c).