Fluorescent probe for rapid measurement of analyte concentration

• US 5,034,189 A
• Filed: 08/27/1985
• Issued: 07/23/1991
• Est. Priority Date: 08/27/1985
• Status: Expired due to Fees

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Parker, Jennifer W., Cox, Mary E.
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): McMahon, Timothy M.
• Application Number:
  US06/769,881
• Time in Patent Office: 2,156 Days
• Field of Search: 436/95, 436/172, 436/163, 422/68, 422/55-60, 435/817
• US Class Current: 422/52
• CPC Class Codes: G01N 2021/6434 Optrodes
• CPC Class Codes: G01N 21/643 non-biological material
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode
• CPC Class Codes: Y10T 436/144444 Glucose

Abstract

A fluorescent optical probe employed for the analysis of the concentration of an analyte (or material to be analyzed) in a fluid, employs a permeable, transparent polymeric matrix in which a fluorophor is immobilized, with the polymeric material being directly exposed to the fluid being analyzed. The composite material of the probe may be made up of a homogeneous matrix of transparent polymer, fluorescent compound, catalyst(s) and reagents(s) and is employed to measure analyte concentration in a fluid in the environment surrounding the material. For analyzing oxygen, the fluorophor may be 9,10-diphenyl anthracene and the polymer matrix may be poly (dimethyl siloxane), or silicone, with the presence of oxygen quenching the fluorescence of 9,10-diphenyl anthracene. For analyzing the concentration of glucose, the polymeric material may be poly (hydroxyethyl methacrylate), or PHEMA, the fluorophor may be 9,10-diphenyl anthracene, and as catalytic material, glucose oxidase may also be immobilized within the PHEMA matrix to reduce the quenching action of the oxygen, with increased output radiation therefore indicating higher levels of glucose. More generally, the fluorophor, and the catalyst, and other reagents, when utilized, are immobilized, either physically or chemically, in a homogeneous manner throughtout the polymer host. Examples of other analytes and catalysts are given.

Claims

1. A system for rapidly detecting the concentration of an analyte in a fluid comprising:

a fluorescent probe;

means for exposing said probe to a fluid to be analyzed;

means for directing exciting illumination into said fluorescent probe;

means for detecting radiation originating in said fluorescent probe and changes in the radiation with variations in the concentration of said analyte;

said probe being formed of a transparent polymeric matrix having a fluorophor immobilized within the body of said polymeric material, said polymeric material being permeable to the substance being analyzed;

said fluorophor being 9,10 diphenyl anthracene;

means for mounting said probe with said polymeric matrix directly exposed to the fluid to be analyzed, whereby the response time of said system is minimized; and

ease of manufacturing and design flexibility are increased;

said polymeric matrix being substantially pure poly (dimethyl siloxane); and

fibrinolytic enzymes included in said polymeric matrix, whereby the formation of blood clots is inhibited.