Reference electrode and a measuring apparatus using the same
First Claim
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1. A silver/silver chloride reference electrode, comprising:
- a silver electrode portion;
a first layer, containing a mixture of silver, silver chloride and protein, on said silver electrode portion, wherein said protein is crosslinked to crosslink said first layer, and wherein said first layer is formed by processing said silver electrode portion electrolytically in an electrolyte solution containing chloride ions and a protein having an isoelectric point lower than the pH of said electrolyte solution to form a thin layer and then treating said thin layer with a crosslinking agent to crosslink said protein to form said first layer; and
a hydrophilic gel layer formed on said first layer such that said first layer can contact an electrolytic solution through said hydrophilic gel layer.
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Abstract
A reference electrode for use in a flow type measuring apparatus incorporating an immobilized enzyme working electrode, wherein a hydrophilic gel layer is provided on a silver chloride containing layer in order to prevent flow-out of silver ions that are likely to adversely affect enzyme activity, so that the silver chloride containing layer goes into contact through the hydrophilic gel layer with an electrolytic solution containing a substance to be measured.
245 Citations
17 Claims
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1. A silver/silver chloride reference electrode, comprising:
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a silver electrode portion; a first layer, containing a mixture of silver, silver chloride and protein, on said silver electrode portion, wherein said protein is crosslinked to crosslink said first layer, and wherein said first layer is formed by processing said silver electrode portion electrolytically in an electrolyte solution containing chloride ions and a protein having an isoelectric point lower than the pH of said electrolyte solution to form a thin layer and then treating said thin layer with a crosslinking agent to crosslink said protein to form said first layer; and a hydrophilic gel layer formed on said first layer such that said first layer can contact an electrolytic solution through said hydrophilic gel layer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A silver/silver chloride reference electrode formed by the steps of:
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providing an electrolytic solution containing chloride ions; dissolving protein having an isoelectric point lower than the pH of said electrolytic solution containing chloride ions in said electrolytic solution; processing a silver electrode electrolytically in said electrolytic solution to form a layer containing a mixture of silver, silver chloride and protein; and treating said formed layer with a crosslinking agent to crosslink the protein in the layer.
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14. A measuring apparatus comprising:
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a silver/silver chloride reference electrode having a first layer containing silver chloride, and a hydrophilic gel layer formed on said first layer, whereby said first layer can contact an electrolytic solution through said hydrophilic gel layer and wherein said first layer is formed by processing a silver electrode electrolytically in an electrolyte solution containing chloride ions and a protein having an isoelectric point lower than the pH of said electrolytic solution, whereby a layer is formed containing a mixture of silver, silver chloride and protein, and then treating the formed layer with a crosslinking agent to crosslink the protein; and an immobilized enzyme working electrode. - View Dependent Claims (15, 16, 17)
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Specification