Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers

  • US 5,043,272 A
  • Filed: 04/27/1989
  • Issued: 08/27/1991
  • Est. Priority Date: 04/27/1989
  • Status: Expired due to Term
First Claim
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1. A process for substantially amplifying template nucleic acid sequences in a sample in a randomly primed and template dependent manner, comprising the steps of:

  • (a) priming template nucleic acid strands with an excess of random oligonucleotide primers; and

    (b) incubating said template nucleic acid strands and said excess random oligonucleotide primers in the presence of an excess of an inducing agent, a strand displacement agent, and an excess of triphosphate substrates to randomly amplify nucleic acid strands.

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