Method for judging errors in applying liquid samples during biochemical analysis

• US 5,051,901 A
• Filed: 08/20/1990
• Issued: 09/24/1991
• Est. Priority Date: 08/21/1989
• Status: Expired due to Term

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Endo, Hirotoshi
• Primary Examiner(s): Smith, Jerry
• Assistant Examiner(s): Brutman, Laura
• Application Number:
  US07/570,078
• Time in Patent Office: 400 Days
• Field of Search: 364/497, 364/413.11, 364/413.08, 364/413.1, 364/552, 356/39
• US Class Current: 702/19
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: G01N 2201/122 Kinetic analysis; determining reaction rate
• CPC Class Codes: G01N 2201/1241 Multirange
• CPC Class Codes: G01N 2201/127 Calibration; base line adjustment; drift compensation
• CPC Class Codes: G01N 33/48 Biological material, e.g. blood, urine G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence; Haemocytometers counting blood corpuscules distributed over a surface by scanning the surface G06M11/02

Abstract

Errors in applying liquid sample are judged during biochemical analysis wherein a droplet of liquid sample is applied to an analysis medium containing a reagent, which chemically reacts with a specific constitutent in the liquid sample, the analysis medium is then incubated, the optical densities of the analysis medium are determined, and concentration of the specific constituent in the liquid sample is determined from the optical densities of the analysis medium thus determined. The method for judging errors in applying a liquid sample comprises the steps of determining the optical density of the analysis medium plural times with the passage of time while the analysis medium is being incubated, and calculating the difference between the maximum value and the minimum value of the optical densities thus determined for the analysis medium. The difference is compared with a predetermined threshold value. In cases the difference is smaller than the threshold value, it is judged that errors occurred in applying a liquid sample to the analysis medium.

Claims

1. A method for judging errors in applying a liquid sample during a biochemical analysis wherein a droplet of liquid sample is applied to an analysis medium containing a reagent, which chemically reacts with a specific constituent in the liquid sample, the analysis medium is then incubated, the optical densities of the analysis medium are determined, and concentration of the specific constituent in the liquid sample is determined from the optical densities of the analysis medium thus determined,

the method for judging errors in applying a liquid sample during a biochemical analysis comprising the steps of:

i) determining the optical density of said analysis medium plural times with the passage of time while said analysis medium is being incubated,

ii) calculating the difference between the maximum value and the minimum value of the optical densities thus determined for said analysis medium,

iii) comparing said difference with a predetermined threshold value, and

iv) in cases said difference is smaller than said threshold value, judging that errors occurred in applying a liquid sample to said analysis medium.

2. A method as defined in claim 1 wherein said analysis medium is a chemical analysis slide.

3. A method as defined in claim 1 wherein said analysis medium is a long test film.

4. A method as defined in claim 1 wherein said optical densities of said analysis medium are determined by use of the formula ##EQU2## where W denotes the measured value representing the amount of light reflected by a white reference plate, B denotes the measured value representing the amount of light reflected by a black reference plate, S denotes the measured value representing the amount of light reflected by the analysis medium, ODw denotes the reflection density of the white reference plate determined with a reference density meter, and ODb denotes the reflection density of the black reference plate determined with a reference density meter.