Method of monitoring reagent delivery in a scanning spectrophotometer
First Claim
1. A method for measuring the concentration of thromboplastin in a reaction mixture comprising the steps of:
- adding dye to thromboplastin until the dye is at given concentration in the thromboplastin;
mixing the thromboplastin with a reaction mixture comprising a blood plasma sample which reacts with the thromboplastin to form a reaction product;
measuring the formation of the reaction product at a first spectral region;
measuring the concentration of dye in the reaction mixture at a second spectral region in which the dye has an optical characteristic, the second spectral region being different from the first spectral region; and
determining the concentration of the thromboplastin in the reaction mixture based on the concentration of dye measured.
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Abstract
A method for measuring the concentration of a reagent in a reaction mixture including the steps of: adding dye to a reagent until the dye is at a given concentration in the reagent; mixing the reagent with a reaction mixture comprising a sample which reacts with the reagent to form a reaction product; measuring the formation of reaction product at a first spectral region; measuring the concentration of dye in the reaction mixture at a second spectral region in which the dye has an optical characteristic such as absorption or fluorescence, the second spectral region being different from the first spectral region; and determining the concentration of the reagent in the reaction mixture based on the concentration of dye measured.
In a further aspect of the invention there is provided a reagent containing a dye useful in the above method.
64 Citations
17 Claims
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1. A method for measuring the concentration of thromboplastin in a reaction mixture comprising the steps of:
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adding dye to thromboplastin until the dye is at given concentration in the thromboplastin; mixing the thromboplastin with a reaction mixture comprising a blood plasma sample which reacts with the thromboplastin to form a reaction product; measuring the formation of the reaction product at a first spectral region; measuring the concentration of dye in the reaction mixture at a second spectral region in which the dye has an optical characteristic, the second spectral region being different from the first spectral region; and determining the concentration of the thromboplastin in the reaction mixture based on the concentration of dye measured. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method for measuring the concentration of a thrombin in a reaction mixture comprising the steps of:
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adding dye to thrombin until the dye is at given concentration in the thrombin; mixing the thrombin with a reaction mixture comprising a blood plasma sample which reacts with the reagent to form a reaction product; measuring the formation of the reaction product at a first spectral region; measuring the concentration of dye in the reaction mixture at a second spectral region in which the dye has an optical characteristic, the second spectral region being different from the first spectral region; and determining the concentration of the thrombin in the reaction mixture based on the concentration of dye measured. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16)
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17. A method for measuring the concentration of a reagent in a reaction mixture comprising the steps of:
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adding rhodamine B to a reagent until the rhodamine B is at given concentration in the reagent; mixing the reagent with a reaction mixture comprising a biological sample which reacts with the reagent to form a reaction product; measuring the formation of the reaction product at a first spectral region; measuring the concentration of rhodamine B in the reaction mixture at a second spectral region in which the rhodamine B fluoresces, the second spectral region being different from the first spectral region; and determining the concentration of the reagent in the reaction mixture based on the concentration of rhodamine B measured.
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Specification