Use of daylight fluorescent pigments for tagging biological molecules

• US 5,075,215 A
• Filed: 06/03/1991
• Issued: 12/24/1991
• Est. Priority Date: 06/11/1984
• Status: Expired due to Term

• Current Assignee: William J. Dreyer
• Original Assignee: William J. Dreyer
• Inventors: Dreyer, William J.
• Primary Examiner(s): YARBROUGH, AMELIA
• Application Number:
  US07/710,375
• Time in Patent Office: 204 Days
• Field of Search: 435/6, 435/803, 435/7.4, 435/91, 436/94, 436/501, 436/518, 436/523, 436/4, 436/800, 436/805, 436/809, 436/810, 436/818, 436/814, 422/61, 935/78, 935/81, 935/76
• US Class Current: 435/6.14
• CPC Class Codes: C12Q 1/6813 Hybridisation assays
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: Y10S 436/80 Fluorescent dyes, e.g. rhodamine
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick
• CPC Class Codes: Y10S 436/814 Pregnancy
• CPC Class Codes: Y10S 436/818 Human chorionic gonadotropin
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

A general method of assay for biological molecules using daylight fluorescent particles. The method described is applicable to assays involving immunological reagents, nucleic acids, hormones and neurotransmitters.

Claims

1. A composition for use in assaying first biological molecules, comprising a plurality of microparticles exhibiting daylight fluorescence bound to second molecules complementary to those being assayed.

2. A composition according to claim 1 where the first and second molecules are immunological ligands.

3. A composition according to claim 1 where the first and second molecules are complementary strands of nucleic acids.

4. A composition for use in assaying of biological molecules including:

(a) a plurality of first molecules complementary to second molecules to be assayed, said second molecules being said biological molecules; and

(b) pigmented daylight fluorescent microparticles coated with third molecules also complementary to the second molecules.

5. A composition according to claim 4 wherein said second molecules are human chorionic gonadotropin.

6. A composition according to claim 4 in which microparticles exhibiting daylight fluorescence are bound to molecules complementary to molecules present on the surfaces of the labeled cells.

7. A composition according to claim 4 in which the complementary molecules are immunological ligands.

8. A composition for use in assaying biological molecules comprising a substrate to which are bound first molecules complementary to second molecules on a portion of the surface of said substrate, said second molecules being said biological molecules, the balance of the surface of said substrate having bound thereto third molecules, similar to the first molecules, but not complementary to the second molecules, and fourth molecules, also complementary to the second molecules and labeled with a daylight fluorescent pigment.

9. A composition according to claim 8 wherein said second molecules are human choronic gonatropin.

10. A composition according to claim 8 wherein the substrate is a convoluted surface.

11. A composition according to claim 8 wherein the complementary molecule is an immunological ligand.

12. A composition according to claim 8 wherein the complementary molecule is a nucleic acid.

13. A composition for use in assaying of biological molecules including:

(a) a plurality of first molecules complementary to second molecules to be assayed, said second molecules being said biological molecules, the first molecules being bound to the surface of a convoluted substrate in a container;

(b) the binding sites of the first molecules are saturated with third molecules which are the second molecules or analogues thereof; and

(c) the third molecules are covalently bound to the surfaces of daylight fluorescent pigmented microparticles.