Device and method for detecting microorganisms

• US 5,094,955 A
• Filed: 02/15/1990
• Issued: 03/10/1992
• Est. Priority Date: 03/15/1988
• Status: Expired due to Term

• Current Assignee: Biomerieux Incorporated (Compagnie Merieux Alliance SAS)
• Original Assignee: Akzo Nobel N.V. (Nouryon BV)
• Inventors: Moody, Carmella S., DiGuiseppi, James L., Thorpe, Thurman C., Turner, James E., Calandra, Michael J., Driscoll, Richard C.
• Primary Examiner(s): Jones, Larry
• Application Number:
  US07/480,398
• Time in Patent Office: 754 Days
• Field of Search: 435/291, 435/289, 435/288, 435/287, 435/284, 435/286, 435/296, 435/34, 435/29, 435/313, 435/807, 435/808, 436/146, 422/68, 422/52
• US Class Current: 435/288.7
• CPC Class Codes: C12M 23/22 Transparent or translucent parts glassware for laboratory use B01L3/00
• CPC Class Codes: C12M 41/26 of pH
• CPC Class Codes: C12M 41/34 of gas
• CPC Class Codes: C12M 41/46 of cellular or enzymatic activity or functionality, e.g. cell viability
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 21/80 Indicating pH value
• CPC Class Codes: Y10S 435/807 Gas detection apparatus
• CPC Class Codes: Y10T 436/235 In an aqueous solution [e.g., TOC, etc.]

Abstract

An instrument and a sealable vessel for detecting the presence of microorganisms in a specimen, the vessel containing a liquid culture medium communicating with a sensor means containing an indicator medium therein. Changes in the indicator medium resulting from pH change or change in CO₂ concentration in the medium are detected from outside the vessel.

Claims

1. A device for continuously monitoring biological activity in a specimen comprising a sealable specimen container with a sealing means, having an internal chamber in which a specimen may be cultured with a culture medium, having at least one transparent section and a sensor means located inside said container in the region of the transparent section, whereby changes in the appearance of the sensor means can be continuously monitored from the exterior of said container through said transparent section, thereby monitoring biological activity without violating the integrity of said container after sealing, said sensor means comprising a membrane and an indicator medium, the indicator medium being selected for its ability to exhibit a detectable change when exposed to products of an organism'"'"'s metabolic activity.

2. The device according to claim 1, wherein the sealing means used to seal said container comprises said transparent section.

3. The device according to claim 1, wherein said sealing means incorporates said sensor means.

4. The device of claim 3, wherein the sensor means comprises a charged membrane.

5. The device of claim 4, wherein the sensor means comprises a positively charged membrane.

6. The device of claim 4, wherein the sensor means comprises a negatively charged membrane.

7. The device of claim 4, wherein the sensor means comprises a positively charged nylon membrane.

8. The device of claim 1 comprising a multiplicity of separate specimen containers, wherein at least one of the containers has a sensor located therein.

9. The device of claim 8, comprising a microtiter plate.

10. The device of claim 8, wherein said sensor is introduced into each container as a liquid emulsion, which is cured in situ.

11. The device of claim 10, wherein the emulsion is introduced into each container by spraying.

12. A device according to claim 1, wherein said sensor means is sprayed onto said transparent section.

13. The device of claim 1, wherein the indicator medium comprises at least one molecular species responsive to pH.

14. The device of claim 13, wherein the indicator medium comprises a combination of molecular species responsive to a range of pH.

15. The device of claim 1, wherein the indicator medium is a molecular species responsive to the presence of NH₃.

16. The device of claim 1, wherein the sensor means comprises a charged membrane.

17. The device of claim 1, wherein the membrane of the sensor means is bonded to the interior surface of said container.

18. The device of claim 17, wherein the membrane of the sensor means is at least circumferentially bonded to the interior surface of the container.

19. The device of claim 1, wherein the sensor means is separated from the specimen in said container by a semipermeable membrane.

20. The device of claim 19, wherein the semipermeable membrane comprises a membrane formed in situ.

21. The device of claim 1, wherein the sensor means comprises droplets of indicator medium immobilized within a polymer matrix and the polymer matrix makes up the membrane.