Diagnosis by means of fluorescent light emission from tissue

• US 5,115,137 A
• Filed: 11/28/1990
• Issued: 05/19/1992
• Est. Priority Date: 02/22/1989
• Status: Expired due to Term

• Current Assignee: Novadaq Technologies, Inc. (Stryker Corporation)
• Original Assignee: Spectraphos AB
• Inventors: Stenram, Unne, Andersson-Engels, Stefan, Svanberg, Sune, Johansson, Jonas, Svanberg, Katarina
• Primary Examiner(s): Hannaher, Constantine
• Application Number:
  US07/598,716
• Time in Patent Office: 538 Days
• Field of Search: 128/634, 128/633, 250/461.2
• US Class Current: 250/461.2
• CPC Class Codes: G01N 2021/3177 Use of spatially separated filters in simultaneous way
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 21/6408 with measurement of decay time, time resolved fluorescence
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging

Abstract

A sample (1) is irradiated by a pulse from a laser (2), creating fluorescence radiation, which is brought, in imaging relation or otherwise, to a detector (6) sensing from the same sample (1) in a plurality of spectral intervals (6A-6D), for obtaining intensity values. The radiation may be sensed in imaging relation, creating a multiplicity of said pluralities. The spectral intervals may be wavelength defined by passband filters (5A-5D) or time interval defined time spectral intervals controlled by a computer (7), which makes a numerical evaluation comprising a division. The tissue character may then be evaluated from the evaluation.

Claims

1. A method for determining tissue character by fluorescence, comprising the steps of

i. exciting the tissue with a laser wavelength below 500 nm,

ii. detecting a fluorescence intensity within a plurality of predetermined spectral intervals for obtaining a plurality of numerical intensity values, at least two of said predetermined spectral intervals comprising spectral intervals centered on wavelengths having substantially equal absorption values in blood,

iii. performing an arithmetic operation on said numerical intensity values including at least one division operation.

2. The method of claim 1, wherein said predetermined spectral intervals comprise wavelength intervals.

3. The method of claim 1, wherein said predetermined spectral intervals include intervals in time, starting and ending in predetermined relationship to the end of a duration of a pulse from said laser.

4. The method of claim 1, wherein said predetermined spectral intervals comprise a plurality of wavelength intervals, each wavelength interval being detected in a predetermined interval in time, starting and ending in predetermined relationship to the end of a duration of a pulse from said laser.

5. The method of claim 1, wherein the tissue is excited with a laser wavelength below 400 nm.

6. The method of claim 1, wherein said detection step comprises simultaneous detection of fluorescence intensity for said plurality of spectral intervals from a multitude of surface elements in a sample illuminated by the said laser pulse.

7. A device for determining tissue character by fluorescence, comprising

i. a laser illumination source,

ii. detector means for detecting fluorescent light from a sample illuminated by said source in a plurality of predetermined spectral fluorescence intervals for obtaining a plurality of intensity values, at least two of said predetermined spectral intervals being centered in wavelength on a pair of wavelength values having substantially equal absorption values in blood,

iii. arithmetic and/or logical means for performing an operation on said plurality of intensity values, including at least one division operation, for obtaining a numerical value characteristic of the tissue character of the sample.

8. A device according to claim 7, comprising spectral resolution means for predetermining detector wavelength intervals for said plurality of spectral intervals.

9. A device according to claim 7, wherein said laser illumination source is a pulsed source, and comprise time resolution means for predetermining time spectral intervals of fluorescence starting and ending in predetermined relationship to pulses from said source.

10. A device according to claim 7, wherein said detector means are multi-channel means for simultaneous detection of fluorescence intensity for said plurality of spectral intervals from a multitude of surface elements in a sample illuminated by the said laser illumination source.