Ice crystal growth suppression polypeptides and method of making

  • US 5,118,792 A
  • Filed: 05/10/1989
  • Issued: 06/02/1992
  • Est. Priority Date: 05/10/1989
  • Status: Expired due to Fees
First Claim
Patent Images

1. A fusion protein consisting essentially of a polypeptide exhibiting ice crystal growth suppression activity and a heterologous protein domain selected from the group consisting of Protein A and β

  • -lactamase, wherein the polypeptide has the formula;

    
    
    space="preserve" listing-type="equation">X1-X2-X3,and is selected from the group consisting of;

    Saf3 wherein X1 is MAA;

    X2 is type 1-type 3-type 3 and X3 is ATAA;

    Saf5 wherein X1 is MAA;

    X2 is type1-type3 -type3-type3-type3 and X3 is ATAA;

    Saf6 wherein X1 is M;

    X2 is type1-type3-type3 and X3 is ATAA;

    Saf8 wherein X1 is M; and

    X2 is type1-type3--type3 and X3 is ATAR; and

    Saf10 wherein X1 is M;

    X2 is type1-type4-type5 and X3 is ATAR;

    and wherein;

    type1 is DTASD AAAAAA,type2 is LTAAN AKAAAE,type3 is LTAAN AAAAAA,type4 is LTAAN AAAAAK,type5 is LTADN AAAAAA,type6 is ATAAT AAAAAA, andtype7 is ATAAT AAKAAA.

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