Apparatus for analysis of specific binding complexes

• US 5,132,097 A
• Filed: 09/04/1990
• Issued: 07/21/1992
• Est. Priority Date: 02/11/1987
• Status: Expired due to Fees

• Current Assignee: G P Research Limited
• Original Assignee: G P Research Limited
• Inventors: Lyon, Jack J., Hillson, Thomas D., Van Deusen, Richard A.
• Primary Examiner(s): WARDEN, JILL ALICE
• Application Number:
  US07/577,802
• Time in Patent Office: 686 Days
• Field of Search: 422/68.1, 422/82.05, 422/82.06, 422/82.07, 422/82.11, 422/82.09, 436/518, 436/164, 436/172, 356/434, 356/443, 356/448
• US Class Current: 422/82.09
• CPC Class Codes: G01N 2021/478 Application in testing analytical test strips
• CPC Class Codes: G01N 2021/5957 using an image detector type detector, e.g. CCD
• CPC Class Codes: G01N 2021/8494 Measuring or storing parameters of the band
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: G01N 2201/0833 Fibre array at detector, resolving
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

A system for analysis of specific binding complexes. A reaction surface is treated with a specific receptor chemical (SRC) having specific binding properties and is allowed to bind with an unknown reactant to form specific binding complexes (SRC) on the reaction surface. The output of a laser is directed through the reaction surface to a detection mechanism. Shadows in the detected light may be interpreted to detect the presence of the unknown reactant. The unknown reactant may also be quantified. Preferred embodiments of apparatus, and methods of their use, are disclosed for detecting the presence of the unknown reactant within a system for interpreting the results, including interactive system components by which the bases for interpretation may be updated.

Claims

1. A detection system for use during analysis of a specific binding complex, the specific binding complex being carried on a reaction surface of a substrate, the detection system comprising:

a source of analytical radiation;

means directing said analytical radiation through said substrate reaction surface while causing said analytical radiation to converge at said reaction surface;

semiconductor means responsive to the analytical radiation passing through said reaction surface for providing a signal representative thereof; and

means for processing said signal.

2. A detection system for use during analysis of a specific binding complex, the specific binding complex being carried on a reaction surface of a substrate, the detection system comprising:

a source of analytical radiation;

means directing said analytical radiation through said substrate reaction surface while causing said analytical radiation to converge at said reaction surface;

means responsive to the analytical radiation passing through said reaction surface for providing a signal representative thereof, said analytical radiation responsive means comprising radiation gathering means and means establishing a pattern representative of gathered radiation wherein said pattern establishing means comprises an array of elements responsive to said analytical radiation; and

said analytical radiation responsive elements comprises semiconductor mean; and

means for processing said signal.