Optical fiber sensors for continuous monitoring of biochemicals and related method

• US 5,143,066 A
• Filed: 05/08/1990
• Issued: 09/01/1992
• Est. Priority Date: 05/08/1990
• Status: Expired due to Fees

• Current Assignee: University Of Pittsburgh (University of Pittsburgh of The Commonwealth System of Higher Education)
• Original Assignee: University Of Pittsburgh (University of Pittsburgh of The Commonwealth System of Higher Education)
• Inventors: Schultz, Jerome S., Komives, Claire
• Primary Examiner(s): Shay, David M.
• Application Number:
  US07/521,081
• Time in Patent Office: 847 Days
• Field of Search: 128/631, 128/633, 128/634, 356/39, 356/418, 356/419, 250/364, 250/365, 422/55
• US Class Current: 600/317
• CPC Class Codes: G01N 2021/6434 Optrodes
• CPC Class Codes: G01N 2021/773 Porous polymer jacket; Polymer matrix with indicator
• CPC Class Codes: G01N 2021/775 Indicator and selective membrane
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B

Abstract

A device and method for measuring properties of certain substances such as analytes are provided. The device includes a probe housing having an optical fiber associated therewith. The housing is capped on one end by a porous membrane which is permeable to the analyte being studied. The housing also includes a reflective surface member disposed between the end of the optical fiber and the membrane, thereby defining a chamber. The reflective surface member does not allow light to be transmitted into or out of the chamber. However, a dye-labeled analog-analyte can permeate the reflective surface member and thereby allow a measurement to be taken which is related to the concentration of the analyte being studied. An embodiment for use in vivo is provided. A related method is also provided.

Claims

1. A sensor for sensing properties of sample analyte, for use with light source means and detection means operatively associated with an optical fiber, comprising:

a porous membrane, permeable to said analyte,

porous reflective surface means adjacent to said porous membrane, for defining a chamber therebetween,

dye-labeled analog-analyte in solution contained in said chamber,

receptor material disposed within said chamber, and said receptor material being capable of chemically binding with said analyte and said analog-analyte, and

transparent retention means wrapped across a wall of said reflective surface means facing out of said chamber such that said transparent retention means traps free dye-labeled analog-analyte which passes through said reflective surface means, and said retention means is transparent to an emission signal generated by fluorescence of said dye-labeled analog-analyte when said dye-labeled analog-analyte is excited with light.

2. A sensor for optically measuring properties of at least one sample analyte, comprising

a probe housing,

optical fiber means for transmitting light energy into and out of said housing, one end of said optical fiber means disposed within a hollow portion of said housing and spaced from one end of said probe housing,

porous membrane means disposed generally adjacent said one end of said housing, for defining a chamber between an inner wall of said porous membrane means and said optical fiber means,

reflective surface means disposed within said chamber between said optical fiber means and said porous membrane means, and spaced from said porous membrane means, said reflective surface means for defining an illuminated region between said one end of said optical fiber means and said reflective surface means and a dark region between said reflective surface means and said porous membrane means,

a dye-labeled analog-analyte in solution disposed within said chamber,

receptor material disposed within either said illuminated region or said dark region, and said receptor material being capable of chemically binding with said analyte and said analog-analyte,

light source means for generating excitation energy, said light source means operatively associated with said optical fiber means such that said excitation light passes through said optical fiber means to said reflective surface means, and

detection means operatively associated with said optical fiber means, for detecting an emission signal generated by said dye-labeled analog-analyte.

3. The sensor of claim 2 wherein said receptor material binds with

said analyte which is water soluble biochemical.

4. The sensor of claim 2 wherein

said receptor material is a substance selected from the group consisting of bioreceptors, enzymes, antibodies and receptor proteins.

5. The sensor of claim 2 wherein said porous membrane means is composed of a material selected from the group consisting of semi-permeable membranes, mobile carrier membranes, and bi-layer membranes.

6. The sensor of claim 2 wherein

said reflective surface means is at least partially composed of noble metal material selected from the group consisting of silver, gold and platinum.

7. The sensor of claim 2 wherein said chamber has seal means for dividing said chamber into separate liquid-tight zones and said reflective surface means has corresponding sections each said section associated with one of said liquid-tight zones.

8. The sensor of claim 7 wherein said liquid-tight zones each contain a different analog-analyte corresponding to separate analytes and said detection means has means for reading a separate emission signal corresponding to each separate analyte.

9. The sensor of claim 7 wherein said liquid-tight zones each correspondence to a different concentration of said analyte.

10. The sensor of claim 2 wherein

said reflective surface means has a porosity such that said analog-analyte may pass therethrough and be detected by said detection means by fluorescent light emitted from said analog-analyte and transmitted as an emission signal through said optical fiber means.

11. The sensor of claim 10 wherein

said porous membrane means has a porosity such that said analyte may diffuse therethrough into said chamber and said porosity being such that diffusion of said dye-labeled analog-analyte out of said chamber is resisted.

12. The sensor of claim 10 wherein said receptor material is deposited onto said inner wall of said porous membrane means.

13. The sensor of claim 10 wherein said receptor material is deposited onto a wall of said reflective surface means which faces into said dark region.

14. The sensor of claim 10 wherein said receptor material is deposited onto a wall of said reflective surface means which faces into said illuminated region.

15. The sensor of claim 10, further comprising means for applying an electrical potential to said reflective surface means.

16. The sensor of claim 10 wherein said analog-analyte comprises a substance bound to the analyte.

17. The sensor of claim 16 wherein said substance is selected from the group consisting of bovine serum albumin, dextran and Immunoglobulin G.