DNA encoding human 5-HT.sub.1D receptors

• US 5,155,218 A
• Filed: 05/08/1990
• Issued: 10/13/1992
• Est. Priority Date: 05/08/1990
• Status: Expired due to Term

• Current Assignee: Lundbeck Research USA, Inc. (Lundbeck Foundation)
• Original Assignee: Neurogenetic Corporation
• Inventors: Branchek, Theresa, Weinshank, Richard L., Hartig, Paul R.
• Primary Examiner(s): Wax, Robert A.
• Assistant Examiner(s): Marschel, Ardin H.
• Application Number:
  US07/520,716
• Time in Patent Office: 889 Days
• Field of Search: 435/6, 435/29, 435/34, 435/69.1, 435/70.3, 435/71.2, 435/91, 435/172.3, 435/240.2, 435/320, 435/820, 436/501, 436/503, 436/86, 436/811, 536/27, 935/4, 935/9, 935/18, 935/19, 935/29, 935/34, 935/36, 935/41, 935/70, 935/72, 935/77, 935/78, 935/88
• US Class Current: 536/23.5
• CPC Class Codes: A01K 2207/15 Humanized animals
• CPC Class Codes: A01K 2217/00 Genetically modified animals
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A01K 2217/075 inducing loss of function, i.e. knock out
• CPC Class Codes: A01K 2227/10 Mammal
• CPC Class Codes: A01K 2267/03 Animal model, e.g. for test or diseases
• CPC Class Codes: A01K 2267/0318 Animal model for neurodegenerative disease, e.g. non- Alzheimer's
• CPC Class Codes: A01K 2267/0362 Animal model for lipid/glucose metabolism, e.g. obesity, type-2 diabetes
• CPC Class Codes: A61K 2039/505 comprising antibodies
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 25/06 Antimigraine agents
• CPC Class Codes: A61P 25/28 for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07K 14/70571 for neuromediators, e.g. serotonin receptor, dopamine receptor
• CPC Class Codes: C07K 16/286 against neuromediator receptors, e.g. serotonin receptor, dopamine receptor
• CPC Class Codes: C12N 15/8509 for producing genetically modified animals, e.g. transgenic
• CPC Class Codes: Y10S 435/82 Subcellular parts of microorganisms
• CPC Class Codes: Y10S 436/811 Test for named disease, body condition or organ function

Abstract

This invention provides isolated nucleic acid molecules encoding human 5-HT_1D receptors, isolated proteins which are human 5-HT_1D receptors, vectors comprising isolated nucleic acid molecules encoding human 5-HT_1D receptors, mammalian cells comprising such vectors, antibodies directed to the human 5-HT_1D receptors, nucleic acid probes useful for detecting nucleic acid encoding human 5-HT_1D receptors, antisense oligonucleotides complementary to any sequences of a nucleic acid molecule which encodes a human 5-HT_1D receptor, pharmaceutical compounds related to human 5-HT_1D receptors, and nonhuman transgenic animals which express DNA a normal or a mutant human 5-HT_1D receptor. This invention further provides methods for determining ligand binding, detecting expression, drug screening, and treatment involving the human 5-HT_1D receptor.

Claims

1. An isolated DNA molecule encoding a human 5HT_1D-1 receptor having the coding sequence shown in FIGS. 3A and 3B.

3. A vector comprising the DNA molecule of claim 1.

4. A plasmid of claim 3.

5. A vector of claim 3 adapted for expression in a bacterial cell which comprises the regulatory elements necessary for expression of the DNA in the bacterial cell so located relative to the DNA encoding the 5HT_1D receptor as to permit expression thereof.

6. A vector of claim 3 adapted for expression in a yeast cell which comprises the regulatory elements necessary for expression of the DNA in the yeast cell so located relative to the DNA encoding the 5-HT_1D receptor as to permit expression thereof.

7. A vector of claim 3 adapted for expression in a mammalian cell which comprises the regulatory elements necessary for expression of the DNA in the mammalian cell so located relative to the DNA encoding the 5-HT_1D receptor as to permit expression thereof.

8. A plasmid of claim 4 adapted for expression in a mammalian cell which comprises the regulatory elements necessary for expression of the DNA in the mammalian cell so located relative to the DNA encoding the 5-HT_1D receptor as to permit expression thereof.

11. A mammalian cell comprising the plasmid of claim 4.

12. An Ltk- cell comprising the plasmid of claim 4.

2. An isolated DNA molecule encoding a human 5-HT_1D-2 receptor having the coding sequence shown in FIGS. 4A and 4B.

9. A plasmid designated pcEXV-8-30-84 (ATCC Accession No. 40790).

13. An Ltk- cell comprising the plasmid of claim 9, designated Ltk-8-30-84 (ATCC Accession No. CRL 10421).

10. A plasmid designated pSVL-11 (ATCC Accession No. 40791).

14. An Ltk- cell comprising the plasmid of claim 10, designated Ltk-11 (ATCC Accession No. CRL 10422).