Device and method for enhanced recovery and detection of microbial growth in the presence of antimicrobial substances
First Claim
1. A device for the enhanced recovery and detection of microorganisms and for continuously monitoring biological activity in a sample comprising a sealable, sterilizable, specimen container, having an internal chamber in which a sample may be cultured, the internal chamber enclosing a sterile culture medium and an adsorbent in an amount that is effective for neutralizing, binding, or inhibiting antimicrobial substances present in said sample and said medium, the container having at least one transparent section therein and a sensor means located inside said container in the region of the transparent section, said sensor means comprising a membrane and an indicator medium, the indicator medium being selected for its ability to exhibit a detectable change when exposed to products of an organism'"'"'s metabolic activity whereby changes in the appearance of the sensor means can be continuously monitored from the exterior of said container through said transparent section, thereby monitoring biological activity without violating the integrity of said container after sealing.
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to a device and method of detecting microorganisms in a rapid manner and increasing the number of microorganisms detected during the culturing of a sample by the addition of resinous and non-resin adsorbents and molecular sieves in the growth media. These agents have been found to neutralize or remove inhibitory and antimicrobial substances found in samples and media.
81 Citations
14 Claims
- 1. A device for the enhanced recovery and detection of microorganisms and for continuously monitoring biological activity in a sample comprising a sealable, sterilizable, specimen container, having an internal chamber in which a sample may be cultured, the internal chamber enclosing a sterile culture medium and an adsorbent in an amount that is effective for neutralizing, binding, or inhibiting antimicrobial substances present in said sample and said medium, the container having at least one transparent section therein and a sensor means located inside said container in the region of the transparent section, said sensor means comprising a membrane and an indicator medium, the indicator medium being selected for its ability to exhibit a detectable change when exposed to products of an organism'"'"'s metabolic activity whereby changes in the appearance of the sensor means can be continuously monitored from the exterior of said container through said transparent section, thereby monitoring biological activity without violating the integrity of said container after sealing.
-
7. A method for neutralizing antimicrobial substances in a culture medium and a sample to enhance the recovery and detection of microorganisms in said sample comprising:
-
a) preparing culture medium, b) adding to said medium at least one non-resinous adsorbent in an amount that is effective for neutralizing, binding or inhibiting antimicrobial substances, and c) adding the sample to be tested to said medium containing said non-resinous adsorbent. - View Dependent Claims (8, 9, 10)
-
Specification