Solid phase peptide synthesis using a polyacrylic support in aqueous solution

• US 5,175,254 A
• Filed: 09/26/1991
• Issued: 12/29/1992
• Est. Priority Date: 09/24/1988
• Status: Expired due to Fees

• Current Assignee: Societe d'Expansion Scientifique Expansia
• Original Assignee: Societe d'Expansion Scientifique Expansia
• Inventors: Mery, Jean, Follet, Michel, Naharisoa, Hanitra, Calas, Bernard
• Primary Examiner(s): Cashion, Jr., Merrell C.
• Assistant Examiner(s): NOT, DEFINED
• Application Number:
  US07/766,899
• Time in Patent Office: 460 Days
• Field of Search: 525/327.1, 525/326.7, 530/333, 530/334
• US Class Current: 530/334
• CPC Class Codes: C07K 1/04 on carriers C07K1/003, C07K1/006 take precedence

Abstract

This invention relates to solid phase peptide synthesis.

Solid phase peptide synthesis is accomplished on polyacrylic resins (such as those described in EP 0 079 812 and EP 0 081 408 which correspond to U.S. Pat. Nos. 4,436,874 and 4,439,545 respectively) by a method which includes in the coupling protocol steps of washing the resin in water and/or aqueous solution(s).

Claims

1. A method for solid phase peptide synthesis, the method comprising attaching a first amino acid residue selected from the twenty naturally occurring L-alpha amino acids to a hydrophilic polyacrylic resin, coupling one or more further amino acid residues of the twenty naturally occurring L-alpha amino acids to form a peptide and detaching the peptide from the resin, characterized in that the coupling of the said amino acid residues to the polyacrylic resin includes steps of washing the resin in water, one or more aqueous solutions, or a combination of the foregoing.

2. The method of claim 1 wherein the hydrophilic polyacrylic resin is a copolymer of three monomers as follows:

(1) a first monomer which provides a matrix for the copolymer and is selected from the group consisting of;

1-(meth)acryloyl-pyrrolidine,

1-(meth)acryloyl-piperidine,

1-(meth)acryoloyl-perhydroazepine,

1-(meth)acryloyl-4-methyl-piperazine,

4-(meth)acryloyl-morpholine,

N,N-dimethyl-(meth)acrylamide and

N,N-diethyl-(meth)acrylamide

(ii) a second monomer which crosslinks the copolymer and is selected from the group consisting of;

N,N'"'"'-di(meth)acroyloyl-diaminomethane and

N,N'"'"'-di(meth)acroyloyl 1-1-diaminoethane, and

(iii) a third monomer which activates the copolymer and is selected from the group consisting of the following acids;

2-(meth)acrylamido-acetic acid,

3-(meth)acrylamido-propionic acid,

4-(meth)acrylamido-butyric acid,

6-(meth)acrylamido-hexanoic acid

N-(meth)acryloyl-L-alanine,

N-(meth)acryloyl-L-valine,

N-(meth)acryloyl-L-leucine,

N-(meth)acryloyl-L-phenylalanine,

N-(meth)acryloyl-L-tyrosine,

N-(meth)acryloyl-L-methionine,

N-(meth)acryloyl-L-lysine,

N-(meth)acryloyl-L-proline

and methyl esters of each of the acids.

3. The method of claim 2 wherein the hydrophilic polyacrylic resin has been amidified with ethylene diamine.

4. The method of any of claims 1, 2 or 3 wherein the N-protecting group used in the amino acid coupling is Boc and the coupling comprises the following steps:

1. Washing;

distilled water--2 to 4 times 2 mn each;

2. Deprotection;

6N HCl in water--once, 2 mn and once again, 30 minutes;

3. Washing;

distilled water--4 to 6 times, 2 mn each;

4. Neutralization;

1 equivalent of borate buffer 12.5 mM pH 8.5-9.0--once, 1 to 2 mn and once again, 1 to 2 mn;

5. Washing;

distilled water--4 to 6 times, 1 to 2 mn each;

6. Washing;

DMF--twice, 1 to 2 mn each;

7. Coupling;

symmetrical anhydride;

8. Washing;

DMF or NMP--twice, 2 mn;

each and

9. Washing;

distilled water--4 times, 2 mn each.

5. The method of any of claims 1, 2 or 3 wherein the N-protecting group used in he amino acid coupling is Fmoc and the coupling comprises the following steps:

1. Washing;

distilled water--4 to 6 times, 2 mn each;

6. Deprotection:

piperidine or diethylamine in water;

3. Washing;

isopropanol, twice, 2 mn each;

distilled water--4 to 6 times, 2 mn each;

4. Optionally washing;

DMF--once, 2 mn;

5. Coupling;

symmetric anhydride, 3 times in excess in DMF, and

6. Washing;

DMF twice, 2 mn each;

distilled water--6 times, 2 mn each.