Dual fiberoptic cell for multiple serum measurements

• US 5,176,882 A
• Filed: 12/06/1990
• Issued: 01/05/1993
• Est. Priority Date: 12/06/1990
• Status: Expired due to Term

• Current Assignee: Pelikan Technologies, Inc.
• Original Assignee: Hewlett-Packard Company (HP Inc.)
• Inventors: Kiang, Teddy, Gray, Damien F., Mauze, Ganapati R.
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): Trembley, Theresa A.
• Application Number:
  US07/624,020
• Time in Patent Office: 761 Days
• Field of Search: 422/82.07, 422/82.04, 422/82.05, 422/82.08, 422/52, 422/82.06, 422/82.09, 128/634, 350/96.29, 356/39, 356/417, 250/458.1, 250/459.1
• US Class Current: 422/82.07
• CPC Class Codes: A61B 5/14539 for measuring pH
• CPC Class Codes: A61B 5/1459 invasive, e.g. introduced into the body by a catheter A61B5/1464 takes precedence
• CPC Class Codes: G01N 2021/6484 Optical fibres
• CPC Class Codes: G01N 2021/772 Tip coated light guide
• CPC Class Codes: G01N 2021/775 Indicator and selective membrane
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53

Abstract

An optical fiber is used in conjunction with a sensor capable of sensing more than one analyte. A first fiber optic sensor cell is used for measuring any combination of ionic species and a second fiber optic sensor cell is used for measuring all gaseous species. In one embodiment, a doped polymer is formed utilizing a hydrophilic polymer which immobilizes a pH sensitive dye and a potassium sensitive fluorescence dye, allowing pH to be measured by detecting the color change of the pH sensitive dye, and the potassium ion concentration to be measured by the change in fluorescence intensity of the potassium sensitive dye. In an alternative embodiment, a doped polymer is formed utilizing a hydrophilic polymer which immobilizes a calcium or sodium ion sensitive fluorescence dye in order to form either a combined pH/calcium sensor or a combined pH/sodium sensor, respectively. Any desired fluorescence dye can be utilized in order to provide detection of a desired analyte or any combination of analytes in conjunction with a pH measurement. A gas sensor is provided utilizing a doped polymer which immobilizes a fluorescence dye sensitive to the gas of interest. In one embodiment, an oxygen sensitive fluorescent dye allows measurement of the partial pressure of oxygen utilizing fluorescence quenching of the oxygen sensitive dye. The concentration of other gases such as nitrous oxide, carbon dioxide, and halogenated anesthetic gases, are measured utilizing infrared absorption within the doped polymer. Each such gas is individually detected utilizing its associated characteristic absorption wavelength.

Claims

1. A physiological sensor comprising:

a sample region for containing a sample including one or more sets of analytes to be determined, each set of analytes including one or more analytes to be determined;

a hydrophilic member located within said sample region;

means for coupling first input energy to said hydrophilic member;

means for coupling first output energy from said hydrophilic member to a detector;

p1 means for causing at least some of said first input energy to be coupled as returning energy to said means for coupling first output energy from said hydrophilic member after passing through a sample, the amount of said returning energy being indicative of pH within said sample region;

dopants contained within said hydrophilic member serving to cause florescence in response to at least some of said first input energy, as a function of one or more analytes of a second set of analytes comprising the set of analytes consisting of one or more ions other than pH, within said sample region;

a hydrophobic member located within said sample region;

means for coupling second input energy to said hydrophobic member;

means for coupling second output energy from said hydrophobic member to a detector;

means for causing at least some of said second input energy to be coupled as returning energy to said means for coupling second output energy from said hydrophobic member after passing through a sample, the amount of said returning energy being indicative of a third set of analytes comprising the set of analytes consisting of one or more gasses other than oxygen; and

dopants contained within said hydrophobilic member serving to cause fluorescence in response to at least some of said second input energy, as a function of oxygen within said sample region.

2. A physiological sensor as in claim 1 wherein said sample region is housed, at least partially, by a membrane which is permeable by said analytes.

3. A physiological sensor as in claim 1 wherein said one or more gases are selected from the group of gases consisting of nitrous oxide, carbon dioxide, and halogenated anesthetic gases.

4. A physiological sensor as in claim 1 wherein said second input energy comprises wavelengths corresponding to one or more characteristic absorption wavelengths of said one or more gases.

5. A physiological sensor as in claim 1 wherein:

said first and second input energy is broadband and covers the region of interest;

said first and second output energy is spectrally separated from said first and second input energy; and

said physiologically sensor further comprises means for analyzing said first and second output energy by multivariant analysis to determine said one or more sets of analytes.

6. A physiological sensor as in claim 5 wherein said means for analyzing said first and second output energy comprises means for analyzing said first and second output energy utilizing singular value decomposition.

7. A physiological sensor as in claim 1 wherein said one or more types of ions are selected from the group of ions consisting of hydrogen, potassium, sodium, calcium, and lithium.

8. An instrument for sensing one or more analytes in a sample comprising:

a sample region for containing a sample including one or more sets of analytes to be determined, each set of analytes including one or more analytes to be determined;

means for providing input energy having a plurality of wavelengths, at least one of said wavelengths corresponding to an associated one of said analytes;

a detector for detecting energy provided by said sample in response to said input energy;

a hydrophilic member located within said sample region;

means for coupling first input energy to said hydrophilic member;

mean for coupling first output energy from said hydrophilic member to said detector;

means for causing at least some of said first input energy to be coupled as returning energy to said means for coupling first output energy from said hydrophilic member after passing through said sample, the amount of said returning energy being indicative of pH within said sample region;

dopants serving to cause fluorescence in response to at least some of said first input energy, as a function of one or more analytes of a second set of analytes comprising the set of analytes consisting of one or more ions other than pH, within said sample region;

a hydrophobic member located within said sample region;

means for coupling second input energy to said hydrophobic member;

means for coupling second output energy from said hydrophobic member to a detector;

means for causing at least some of said second input energy to be coupled as returning energy to said means for coupling second output energy from said hydrophobic member after passing through said sample, the amount of said returning energy being indicative of a third set of analytes comprising the set of analytes consisting of one or more gasses other than oxygen; and

dopants serving to cause fluorescence in response to at least some of said second input energy, as a function of oxygen within said sample region.

9. An instrument as in claim 8 wherein said sample region is housed, at least partially, by a membrane which is permeable by said analytes.

10. An instrument as in claim 8, wherein said one or more gases are selected from the group of gases consisting of oxygen, nitrous oxide, carbon dioxide, and halogenated anesthetic gases.

11. An instrument as in claim 8 wherein said input energy comprises wavelengths corresponding to one or more characteristic absorption wavelengths of said one or more gases.

12. An instrument as in claim 8 wherein said one or more types of ions are selected from the group of ions consisting of hydrogen, potassium, sodium, calcium, and lithium.