Method of reducing neuronal damage using omega conotoxin peptides
First Claim
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1. A method of reducing neuronal damage related to an ischemic condition affecting the central nervous system in a mammalian subject, comprising:
- administering to the subject in a pharmaceutically effective amount, an omega conotoxin (OCT) peptide, including a fragment thereof, effective (i) to bind to an omega conotoxin binding protein, with a binding constant which is within the range of binding affinities of MVIIA, MVIIB, GVIA, GVIIA and RVIA omega conotoxins, (ii) to inhibit voltage-gated calcium currents selectively in neuronal tissue with an ED50 value which is within the range of ED50 values of MVIIA, MVIIB, GVIA, GVIIA and RVIA omega conotoxins, and (iii) to inhibit neurotransmitter release selectively in neuronal tissue, with an IC50 which is within the range of MVIIA, MVIIB, GVIA, GVIIA and RVIA omega conotoxins.
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Abstract
A partially purified omega conotoxin binding protein is disclosed. The protein, either in partially purified form or in a synaptosomal preparation, is useful in identifying compounds for use in reducing neuronal damage related to an ischemic condition, such as stroke, in a human patient.
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4 Claims
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1. A method of reducing neuronal damage related to an ischemic condition affecting the central nervous system in a mammalian subject, comprising:
administering to the subject in a pharmaceutically effective amount, an omega conotoxin (OCT) peptide, including a fragment thereof, effective (i) to bind to an omega conotoxin binding protein, with a binding constant which is within the range of binding affinities of MVIIA, MVIIB, GVIA, GVIIA and RVIA omega conotoxins, (ii) to inhibit voltage-gated calcium currents selectively in neuronal tissue with an ED50 value which is within the range of ED50 values of MVIIA, MVIIB, GVIA, GVIIA and RVIA omega conotoxins, and (iii) to inhibit neurotransmitter release selectively in neuronal tissue, with an IC50 which is within the range of MVIIA, MVIIB, GVIA, GVIIA and RVIA omega conotoxins. - View Dependent Claims (2, 3, 4)
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