Integral multilayer analytical element for analysis of ammonia-forming substrate
First Claim
1. An integral multilayer analytical element for the analysis of an ammonia-forming substrate in a liquid sample containing intrinsic ammonia comprising:
- (I) a light-transmissive, liquid-impermeable support;
(II) an ammonia indicator layer containing a reagent capable of undergoing a detectable change by gaseous ammonia;
(III) a liquid barrier layer which allows gaseous ammonia passing therethrough;
(IV) an ammonia-forming substrate reaction layer containing a reagent capable of reacting with an ammonia-forming substrate to form gaseous ammonia;
(V) an intrinsic ammonia trapping layer having a thickness in the range of 1 to 20 μ
m and containing a buffering reagent therein to keep its pH value in the range of from 7.0 to 8.5 and containing a combination of NADH or NADPH, α
ketoglutaric acid and glutamate dehydrogenase to chemically react with the intrinsic ammonia to convert it into other chemical substance so that the intrinsic ammonia is fixed therein and kept from reaching said reaction layer;
(VI) a porous spreading layer, in a laminate form in this order with the proviso that said (I) is the lowest member and said (VI) is the uppermost layer.
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Abstract
An integral multilayer analytical element for the analysis of an ammonia-forming substrate comprising:
(I) a light-transmissive, liquid-impermeable support:
(II) an ammonia indicator layer containing a reagent capable of undergoing a detectable change by gaseous ammonia;
(III) a liquid barrier layer which allows gaseous ammonia passing therethrough;
(IV) an ammonia-forming substrate reaction layer containing a reagent capable of reacting with an ammonia-forming substrate to form gaseous ammonia;
(V) an intrinsic ammonia trapping layer containing a reagent which acts on intrinsic ammonia to convert it into a form which can not reach said reaction layer; and
(IV) a porous spreading layer, in a laminate form.
84 Citations
7 Claims
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1. An integral multilayer analytical element for the analysis of an ammonia-forming substrate in a liquid sample containing intrinsic ammonia comprising:
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(I) a light-transmissive, liquid-impermeable support; (II) an ammonia indicator layer containing a reagent capable of undergoing a detectable change by gaseous ammonia; (III) a liquid barrier layer which allows gaseous ammonia passing therethrough; (IV) an ammonia-forming substrate reaction layer containing a reagent capable of reacting with an ammonia-forming substrate to form gaseous ammonia; (V) an intrinsic ammonia trapping layer having a thickness in the range of 1 to 20 μ
m and containing a buffering reagent therein to keep its pH value in the range of from 7.0 to 8.5 and containing a combination of NADH or NADPH, α
ketoglutaric acid and glutamate dehydrogenase to chemically react with the intrinsic ammonia to convert it into other chemical substance so that the intrinsic ammonia is fixed therein and kept from reaching said reaction layer;(VI) a porous spreading layer, in a laminate form in this order with the proviso that said (I) is the lowest member and said (VI) is the uppermost layer. - View Dependent Claims (2, 3, 4)
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5. An integral multilayer analytical element for the analysis of an ammonia-forming substrate in a liquid sample containing intrinsic ammonia comprising:
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(I) a light-transmissive, liquid-impermeable support; (II) an ammonia indicator layer containing a reagent capable of undergoing a detectable change by gaseous ammonia; (III) a liquid barrier layer which allows gaseous ammonia passing therethrough; (IV) an ammonia-forming substrate reaction layer containing a reagent capable of reacting with an ammonia-forming substrate to form gaseous ammonia; (V) an intrinsic ammonia trapping layer having a thickness in the range of 1 to 20 μ
m, containing a buffering reagent therein to keep its pH value in the range of from 7.0 to 8.5 and containing a combination of aspartase and fumaric acid or fumarate to chemically react with the intrinsic ammonia to convert it into another chemical substance so that the intrinsic ammonia is fixed therein and kept from reaching said reaction layer;(VI) a porous spreading layer, in a laminate form in this order with the proviso that said (I) is the lowest member and said (VI) is the uppermost layer. - View Dependent Claims (6, 7)
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Specification