Sample measuring method using agglomeration reaction of microcarriers

• US 5,198,369 A
• Filed: 04/19/1991
• Issued: 03/30/1993
• Est. Priority Date: 04/25/1990
• Status: Expired due to Fees

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Saitou, Atsushi, Touge, Yoshiyuki, Itoh, Michiyo, Itoh, Yuuji, Yamazaki, Tatsuya
• Primary Examiner(s): Saunders, David
• Assistant Examiner(s): Chin, Christopher L.
• Application Number:
  US07/687,627
• Time in Patent Office: 711 Days
• Field of Search: 73/53, 356/38, 356/39, 356/318, 356/337-343, 422/82.05, 436/534, 436/536, 436/538, 436/541, 436/805, 435/808
• US Class Current: 436/534
• CPC Class Codes: G01N 21/82 producing a precipitate or turbidity
• CPC Class Codes: G01N 33/53 Immunoassay; Biospecific binding assay; Materials therefor
• CPC Class Codes: G01N 33/54313 the carrier being characterised by its particulate form
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 436/805 Optical property

Abstract

A mixture is prepared by mixing carriers to which a substance specifically reacting on an object substance adheres with a sample containing the object substance. The mixture is irradiated by light having an intensity gradient to concentrate the carriers at and near the irradiated positions by the optical pressure of the light. The efficiency of an agglomeration reaction of the carriers is thereby increased. A qualitative or quantitative measurement of the object substance is performed by detecting a state of agglomeration of the carriers within the mixture.

Claims

1. A sample processing method comprising:

preparing a mixture by mixing carriers to which is adhered a substance that specifically binds to an object substance, with a sample containing said object substance; and

directing light having an intensity gradient onto said mixture to concentrate said carriers at positions irradiated by the light, thereby increasing the efficiency of an agglomeration reaction of said carriers.

2. A method according to claim 1, further comprising the step of relatively changing the position irradiated by the light in said mixture.

3. A method according to claim 1, wherein said light comprises a laser light.

4. A method according to claim 1, wherein said carriers comprise insoluble fine particles.

5. A method according to claim 4, wherein said insoluble fine particles comprise latex particles.

6. A method according to claim 1, wherein said substance adhered to the carriers comprises an antibody specific for an object antigen.

7. A method according to claim 1, wherein said sample comprises a blood sample.

8. A sample measuring method comprising:

preparing a mixture by mixing carriers to which is adhered a substance that specifically binds to an object substance, with a sample containing said object substance;

directing light having an intensity gradient onto said mixture to concentrate said carriers at positions irradiated by the light, thereby increasing the efficiency of an agglomeration reaction of said carriers; and

detecting a state of agglomeration of the carriers within said mixture to perform a measurement of the object substance.

9. A method according to claim 8, wherein the detection of said state of agglomeration comprises optical detection.

10. A method according to claim 8, wherein said light comprises a laser light.

11. A method according to claim 8, wherein said carriers comprise latex particles.

12. A method according to claim 8 wherein said substance adhered to the carriers comprises an antibody specific for an object antigen.

13. A sample measuring method comprising:

preparing a mixture by mixing carriers, to which is adhered a substance that specifically binds to an object substance, with a sample containing said object substance;

directing light having an intensity gradient onto said mixture to concentrate said carriers at positions irradiated by the light, thereby increasing the efficiency of an agglomeration reaction of said carriers; and

detecting a state of agglomeration of the carriers within said mixture, to perform a measurement of said object substance, wherein said detecting comprises obtaining image information at the completion of the agglomeration reaction for image analysis.

14. A method according to claim 13, wherein the detection of said image information comprises detecting an image of the sample by a photosensor array.

15. A method according to claim 13, wherein the detection of said image information comprises detecting an image by performing two-dimensional optical scanning of the sample.

16. A method according to claim 13, wherein the analysis of said image information uses an image obtained by binary-coding a detected image with predetermined threshold value.

17. A method according to claim 13, wherein the analysis of said image information comprises calculating the degree of said agglomeration by calculating an autocorrelation of a detected image.

18. A method according to claim 13, wherein the analysis of said image information comprises calculating a degree of said agglomeration by extracting contours of a detected image.

19. A method according to claim 13, further comprising the step of changing the positions irradiated by the light in said mixture.

20. A method according to claim 13, wherein said light comprises a laser light.