DNA encoding bactericidal/permeability-increasing proteins

• US 5,198,541 A
• Filed: 12/11/1991
• Issued: 03/30/1993
• Est. Priority Date: 08/11/1987
• Status: Expired due to Term

• Current Assignee: New York University
• Original Assignee: New York University
• Inventors: Weiss, Jerrold, Elsbach, Peter
• Primary Examiner(s): Nucker, Christine M.
• Assistant Examiner(s): SIDBERRY, HAZEL
• Application Number:
  US07/805,031
• Time in Patent Office: 475 Days
• Field of Search: 536/27, 435/69.1, 435/71.1, 530/350
• US Class Current: 435/69.1
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 38/1751 Bactericidal/permeability-increasing protein [BPI]
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: C07K 14/4742 Bactericidal/Permeability-increasing protein [BPI]
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

A purified, isolated DNA sequence encoding human bactericidal permeability-increasing protein and biologically-active fragments thereof is provided.

Claims

1. An isolated DNA sequence encoding human bactericidal/permeability-increasing protein or a biologically active polypeptide fragment thereof comprising from about residue 1 to about residue 210.

2. A cDNA sequence according to claim 1.

3. A genomic DNA sequence according to claim 1.

4. A eukaryotic host cell stably transformed or transfected with an isolated DNA sequence according to claim 1.

5. A method for producing a human bactericidal/permeability-increasing protein or a biologically active polypeptide fragment thereof comprising:

(a) growing host cell according to claim 4 in a suitable culture medium; and

(b) isolating the desired human bactericidal/permeability-increasing protein or biologically active polypeptide fragment thereof from said host cells or the medium of their growth.

6. An isolated DNA sequence encoding bactericidal/permeability-increasing protein or a biologically active polypeptide fragment thereof comprising from about residue 1 to about residue 210 selected from the group consisting of:

(a) a DNA sequence according to FIG. 5;

(d) a DNA sequence which encodes bactericidal/permeability increasing protein or a biologically active polypeptide fragment thereof and which hybridizes to the DNA sequence of (a) under stringent hybridization option; and

(c) a DNA sequence which encodes the same bactericidal/permeability increasing protein or a biologically active polypeptide fragment thereof as a DNA sequence of (a) or (b).

7. A eukaryotic host cell transformed or transfected with an isolated DNA sequence according to claim 6.

8. A method for producing bactericidal/permeability-increasing protein or a biologically polypeptide fragment thereof comprising:

(a) growing host cells according to claim 7 in a suitable culture medium; and

(b) isolating the desired human bactericidal/permeability-increasing protein or biologically active polypeptide fragment thereof from said host cells or the medium of their growth.