Enzyme sensor and method of manufacturing the same
First Claim
1. An enzyme sensor comprising:
- an electrically conductive substrate;
a reduction function layer covering at least part of said electrically conductive substrate, including an electron movement medium with a structure represented as [Ru(NH3)6 ]3+ or [Ru(NH3)5 X], where X represents pyridine, halogen ion, nicotinamide or crystal water, and di-valent iron ions, and capable of causing a reduction reaction of hydrogen peroxide; and
an enzyme-fixed layer covering at least part of said reduction function layer.
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Abstract
An enzyme sensor is disclosed, which has a two-layer fixed film structure consisting of a reduction function layer including an electron movement medium and di-valent iron ions and an enzyme-fixed layer covering said reduction function layer, and in which hydrogen peroxide generated through decomposition of glucose or the like in the enzyme-fixed layer is reduced by electron movement medium and di-valent iron ions in the reduction function layer.
Thus, the construction of enzyme sensor is simplified, measurement is extremely facilitated, and the measurement time is reduced.
As a different principle of enzyme sensor, an electrically conductive substrate of conductive carbon or the like is covered with an oxygen-reducing catalytic function layer and enzyme layer.
Thus, there is no problem of contamination of enzyme sensor, and it is possible to readily manufacture and miniaturize enzyme sensors.
547 Citations
4 Claims
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1. An enzyme sensor comprising:
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an electrically conductive substrate; a reduction function layer covering at least part of said electrically conductive substrate, including an electron movement medium with a structure represented as [Ru(NH3)6 ]3+ or [Ru(NH3)5 X], where X represents pyridine, halogen ion, nicotinamide or crystal water, and di-valent iron ions, and capable of causing a reduction reaction of hydrogen peroxide; and an enzyme-fixed layer covering at least part of said reduction function layer.
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2. An enzyme sensor comprising:
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an electrically conductive substrate comprising a carbon fiber with a sectional area of 7.85×
10-7 cm2,an oxygen electrode layer covering said electrically conductive substrate and including a coordinator compound having an oxygen-reducing catalytic function; and an enzyme-fixed layer covering said oxygen electrode layer. - View Dependent Claims (3, 4)
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Specification