Optical glucose sensor apparatus and method

• US 5,209,231 A
• Filed: 11/02/1990
• Issued: 05/11/1993
• Est. Priority Date: 11/02/1990
• Status: Expired due to Fees

• Current Assignee: University of Connecticut (State of Connecticut)
• Original Assignee: University of Connecticut (State of Connecticut)
• Inventors: Cote, Gerard L., Fox, Martin D., Northrop, Robert B.
• Primary Examiner(s): Cohen, Lee S.
• Assistant Examiner(s): NASSER, ROBERT L
• Application Number:
  US07/608,251
• Time in Patent Office: 921 Days
• Field of Search: 128/633, 128/634, 128/665, 128/665, 128/666, 356/364, 356/367, 356/368, 356/369, 356/41
• US Class Current: 600/310
• CPC Class Codes: A61B 3/10 Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions
• CPC Class Codes: A61B 5/14507 specially adapted for measuring characteristics of body fluids other than blood devices for taking samples of body liquids A61B10/0045
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/14558 by polarisation
• CPC Class Codes: G01N 21/21 Polarisation-affecting properties G01N21/19 takes precedence

Abstract

An optically based apparatus for non-invasively determining the concentration of optically active substances in a specimen comprises, a source of a beam of spatially coherent light which is acted upon to produce a rotating linear polarized vector therein. A beam splitter splits the beam into a reference beam and a detector beam for passage through the specimen. The detector beam is received upon exiting the specimen and compared with the reference beam to determine the amount of phase shift produced by passage through the specimen. This amount of phase shift is converted into concentration of the optically active substance in the specimen.

Claims

1. An optically based apparatus for in vivo non-invasively determining concentration of optically active substances in an animal comprising:

(a) a laser for producing a laser beam of spatially coherent light;

(b) means for acting upon said laser beam to produce a rotating linear polarized vector therein including

(i) a linear polarizer for linearly polarizing the laser beam,

(ii) a circular polarizer for circularly polarizing the linearly polarized beam, and

(iii) a rotating linear polarizer for producing a constant amplitude time varying linear polarization in the beam;

(c) beam splitter means for splitting the beam into a reference beam and a detector beam for passage through a specimen;

(d) beam transport means for transporting the detector beam to a selected area of an animal for passage through a medium in the animal containing an optically active substance;

(e) means for receiving the detector beam upon exiting the animal;

(f) means comparing the reference beam with the received detector beam to determine the amount of phase shift produced by rotation of the polarized light in the detector beam during passage through the animal; and

(g) means for converting the amount of phase shift determined by said rotation of the polarized light in the detector beam into a concentration value of the optically active substance in the medium through which said detector beam has passed.

2. The optically based apparatus for non-invasively determining concentration of optically active substances in accordance with claim 1 further comprising means passing the reference beam to a stationary linear polarizer.

3. The optically based apparatus for non-invasively determining concentration of optically active substances in accordance with claim 1 wherein said beam transport means includes optical fibers.

4. The optically based apparatus for non-invasively determining concentration of optically active substances in accordance with claim 1 wherein there is included means for passing the beam through an animal at least two times to increase the path length therethrough.

5. The optically based apparatus for non-invasively determining concentration of optically active substance in accordance with claim 1 wherein there is included means for electronically modulating the light beam to null the observed phase shift resulting from elements other than the optically observed active substance in one of the light beams being compared, thereby providing a closed loop system.

6. The optically based apparatus for non-invasively determining concentration of optically active substances in accordance with claim 5 wherein the light beam which is nulled is the reference beam.

7. The optically based apparatus for non-invasively determining concentration of optically active substances in accordance with claim 5 wherein said electronic modulating means is a Faraday rotary compensator.

8. The optically based apparatus for non-invasively determining concentration of optically active substances in accordance with claim 1 wherein there is included means for nulling the observed phase shift by interposing means for electronic modification of the modality of the light beam.

9. A method for in vivo non-invasively sensing concentration of optically active substance in an animal comprising:

(a) generating a laser beam of spatially coherent light;

(b) producing a rotating linear polarized vector in said light beam by;

(i) passing said light beam through a linear polarizer to linearly polarize said beam;

(ii) passing said linearly polarized beam through a circular polarizer to circularly polarize said beam; and

(iii) passing said circularly polarized beam through a rotating linear polarizer to produce a beam with a constant amplitude time varying linear polarization;

(c) splitting said beam into a reference beam and a detector beam for passage;

(d) conveying said detector beam to a selected area of an animal;

(e) passing said detector beam through a portion of the animal having a medium containing an optically active substance to be measured;

(f) receiving the detector beam exiting said animal;

(g) comparing the received detector beam with said reference beam to determine the amount of phase shift produced by rotation of the polarized light in the detector beam during passage through said medium; and

(h) converting the amount of phase shift determined by said rotation of the polarized light in the detector beam into concentration of the optically active substance in said medium through which said detector beam has passed.

10. The method of sensing concentration of an optically active substance in an animal in accordance with claim 9 including the step of passing said reference beam through a stationary linear polarizer.

11. The method of sensing concentration of an optically active substance in an animal in accordance with claim 9 wherein said method includes the step of passing said detector beam through a beam transport system from said splitter to the animal.

12. The method of sensing concentration of an optically active substance in an animal in accordance with claim 9 wherein said detector beam is passed through the aqueous humor in the anterior chamber of the eye of said animal and wherein said conversion determines the concentration of glucose as said optically active substance.

13. The method of sensing concentration of an optically active substance in an animal in accordance with claim 9 wherein said method includes the step of passing said detector beam through said animal at least two times to increase the length of its path therethrough.

14. The method of sensing concentration of an optically active substance in an animal in accordance with claim 9 wherein there is included the step of electronically modulating one of the light beams to null the observed phase shift in said one of the light beams being compared, thereby providing a closed loop system.

15. The method of sensing concentration of an optically active substance in an animal in accordance with claim 14 wherein the electronically modulating step nulls the reference beam.

16. The method of sensing concentration of an optically active substance in an animal in accordance with claim 14 wherein the step of electronic modulation is effected by use of a Faraday rotary compensator.

17. The method of sensing concentration of an optically active substance in an animal in accordance with claim 14 wherein there is included the step of nulling the observed phase shift by electronically modifying the modality of the light beam.

18. A closed loop optically based apparatus for non-invasively determining concentration of optically active substance in a specimen which eliminates light beam intensity as a variable comprising:

(a) a laser for producing a beam of spatially coherent light;

(b) means for acting upon said light beam to produce a rotating linear polarized vector therein;

(c) beam splitter means for splitting the beam into a reference beam and a detector beam for passage;

(d) means for passing said detector beam through a specimen;

(e) means for receiving the detector beam upon exiting the specimen;

(f) means comparing the reference beam with the received detector beam to determine the amount of phase shift produced by passage through the specimen;

(g) means for electronically modulating the reference light beam to null the observed phase shift resulting from elements other than the optically observed active substance; and

(h) means for converting the amount of phase shift determined into a concentration value of the optically active substance in the specimen.