Sensing surfaces capable of selective biomolecular interactions, to be used in biosensor systems
First Claim
1. A sensing surface suitable for use in a biosensor, comprising:
- a film, having two faces, of a free electron metal selected from the group consisting of copper, silver, aluminum and gold; and
a densely packed monolayer of an organic molecule X-R-Y coated on one of the faces of said film where X is a group selected from the group consisting ofasymmetrical or symmetrical disulfide (-SSR'"'"'Y'"'"', -SSRY), sulfide (-SR'"'"'Y'"'"', -SRY), diselenide (-SeSeR'"'"'Y'"'"', -SeSeRY), selenide (SeR'"'"'Y'"'"', -SeRY),thiol (-SH), isonitrile, nitro (-NO2), selenol (-SeH), trivalent phosphorous compounds, isothiocyanate, xanthate, thiocarbamate, phosphine,thio acid and dithio acid (-COSH, -CSSH)where R and R'"'"' are hydrocarbon chains, optionally interrupted by hetero atoms, of a length exceeding 10 atoms, wherein in the case of an asymmetrical molecule, R'"'"' or R may be H, and Y and Y'"'"' are active groups for covalently binding ligands or a biocompatible porous matrix.
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Accused Products
Abstract
A sensing surface suitable for use in a biosensor, comprising:
a film of a free electron metal selected from the group consisting of copper, silver, aluminum and gold; and
a densely packed monolayer of an organic molecule X-R-Y coated on one of the faces of said film where X is a group selected from the group consisting of
asymmetrical or symmetrical disulfide (--SSR'"'"'Y'"'"', -SSRY, sulfide (-SR'"'"'Y'"'"', -SRY), diselenide (-SeSeR'"'"'Y'"'"', -SeSeRY), selenide (SeR'"'"'Y'"'"', -SeRY),
thiol (-SH), isonitrile, nitro (-NO2), selenol (-SeH), trivalent phosphorus compounds, isothiocyanate, xanthate, thiocarbamate, phosphine,
thio acid and dithio acid (-COSH, -CSSH)
where R and R'"'"' are hydrocarbon chains optionally interrupted by hetero atoms, of a length exceeding 10 atoms, wherein in the case of an asymmetrical molecule, R'"'"' or R may be H, and Y and Y'"'"' are active groups for covalently binding ligands or a biocompatible porous matrix.
541 Citations
27 Claims
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1. A sensing surface suitable for use in a biosensor, comprising:
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a film, having two faces, of a free electron metal selected from the group consisting of copper, silver, aluminum and gold; and a densely packed monolayer of an organic molecule X-R-Y coated on one of the faces of said film where X is a group selected from the group consisting of asymmetrical or symmetrical disulfide (-SSR'"'"'Y'"'"', -SSRY), sulfide (-SR'"'"'Y'"'"', -SRY), diselenide (-SeSeR'"'"'Y'"'"', -SeSeRY), selenide (SeR'"'"'Y'"'"', -SeRY), thiol (-SH), isonitrile, nitro (-NO2), selenol (-SeH), trivalent phosphorous compounds, isothiocyanate, xanthate, thiocarbamate, phosphine, thio acid and dithio acid (-COSH, -CSSH) where R and R'"'"' are hydrocarbon chains, optionally interrupted by hetero atoms, of a length exceeding 10 atoms, wherein in the case of an asymmetrical molecule, R'"'"' or R may be H, and Y and Y'"'"' are active groups for covalently binding ligands or a biocompatible porous matrix. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A sensing element suitable for use in a biosensor, comprising:
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a substrate; a film of a free electron metal selected from the group consisting of copper, silver, aluminum and gold disposed on said substrate, said film having a first and second major surface, said first major surface being in contact with said substrate; and a densely packed monolayer of an organic molecule X-R-Y coated on said second major surface of said film where X is a group selected from the group consisting of asymmetrical or symmetrical disulfide (-SSR'"'"'Y'"'"', -SSRY), sulfide (-SR'"'"'Y'"'"', -SRY), diselenide (-SeSeR'"'"'Y'"'"', -SeSeRY), selenide (SeR'"'"'Y'"'"', -SeRY), thiol (-SH), isonitrile, nitro (-NO2), selenol (-SeH), trivalent phosphorus compounds, isothiocyanate, xanthate, thiocarbamate, phosphine, thio acid and dithio acid (-COSH, -CSSH), where R and R'"'"' hydrocarbon chains, optionally interrupted by hetero atoms, of a length exceeding 10 atoms, wherein in the case of an asymmetrical molecule R'"'"' or R may be H, and Y and Y'"'"' are active groups for covalently binding ligands or a biocompatible porous matrix. - View Dependent Claims (23, 24, 25)
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26. A sensing surface suitable for use in a biosensor comprising:
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a film, having two faces, of a free electron metal selected from the group consisting of copper, silver, aluminum and gold; and
a monolayer of an organic molecule X-R-Y coated on one of the faces of said film where X is a group selected from the group consisting ofasymmetrical or symmetrical disulfide (-SSR'"'"'Y'"'"', -SSRY), sulfide (-SR'"'"'Y'"'"', -SRY), diselenide (-SeSeR'"'"'Y'"'"', -SeSeRY), selenide (SeR'"'"'Y'"'"', -SeRY), thiol (-SH), isonitrile, nitro (-NO2), selenol (SeH), trivalent phosphorous compounds isothiocyanate, xanthate, thiocarbamate, phosphine, thio acid and dithio acid (-COSH, -CSSH) where R and R'"'"' are hydrogen chains, optionally interrupted by hetero atoms, of a length exceeding 10 atoms, wherein in the case of an asymmetrical molecule, R'"'"' or R may be H, Y and Y'"'"' are active groups for covalently binding ligands or a biocompatible porous matrix. - View Dependent Claims (27)
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Specification