Reactor for carrying out biological reactions by means of biocatalysts

• US 5,246,855 A
• Filed: 02/25/1991
• Issued: 09/21/1993
• Est. Priority Date: 01/16/1991
• Status: Expired due to Term

• Current Assignee: Vogelbusch GmbH
• Original Assignee: Vogelbusch GmbH
• Inventors: Katinger, Hermann W. D., Reiter, Manfred, Bluml, Gerald, Zach, Nicolaus, Gaida, Theodor
• Primary Examiner(s): Wityshyn, Michael G.
• Assistant Examiner(s): Beisner, William H.
• Application Number:
  US07/660,696
• Time in Patent Office: 939 Days
• Field of Search: 435/284-286, 435/288, 435/289, 435/290, 435/291, 435/299, 435/310, 435/311, 435/313-316, 435/813, 435/818, 422/140, 422/224, 422/225, 422/227, 422/269, 422/274, 210/150, 210/151, 210/615-617, 210/629, 210/263, 366/101-103, 366/106, 366/107, 261/122
• US Class Current: 435/286.1
• CPC Class Codes: C12M 23/24 Gas permeable parts
• CPC Class Codes: C12M 23/34 Internal compartments or partitions
• CPC Class Codes: C12M 25/14 Scaffolds; Matrices in general C12N5/0068
• CPC Class Codes: C12M 25/18 Fixed or packed bed
• CPC Class Codes: Y10S 435/813 Continuous fermentation

Abstract

A reactor for carrying out biological reactions by means of biocatalysts includes a base which houses an agitator unit and suitable monitor and control devices and a securely attachable lid. Interposed between the lid and the base is an intermediate part in form of a vat which for application as fluid-bed reactor and/or fixed-bed reactor includes at its lower end a gas bubbles-permeable support for a biocatalyst-laden matrix or biocatalyst matrix. Extending from the upper area of the intermediate part toward the base is a circulation line which cooperates with the agitator unit which is designed as circulating pump.

Claims

1. A modular reactor for carrying out biological reactions by means of biocatalysts;

comprising;

a base having an interior space and defining an axis;

a lid;

an intermediate section adapted for providing a reaction zone, with said lid constructed so as to be detachably secured to said intermediate section, said intermediate section being constructed so as to be detachably secured between said base and said lid and including a gas bubbles-permeable support member at the junction to said base, said intermediate section being part of a modular kit so as to be replaceable by another intermediate section for allowing selective application of the reactor as a fluidized bed reactor and fixed bed reactor;

a carrier matrix contained in the reaction zone within said intermediate section above said support member for immobilizing biocatalysts;

circulation means for circulating a nutrient medium between said interior space and said reaction zone and including a circulation pipe comprised of a pipe traversing said intermediate section in axial direction and an extension pipe provided in said interior space of said base and detachably secured to said pipe in said intermediate section, and an agitating means accommodated in said base for circulating the nutrient medium through said circulation pipe; and

control means arranged in said base for monitoring and controlling conditions in the reactor for each mode of operation of the reactor.

2. A reactor as defined in claim 1 wherein said support member includes a support plate with a hydrophobic surface and a permeability of 3 to 12%.

3. A reactor as defined in claim 2 wherein said support plate has a permeability of 5 to 7.5%.

4. A reactor as defined in claim 1 wherein said gas bubbles-permeable support member is a static mixer for creating an increased oxygen transfer during flow of nutrient medium therethrough.

5. A reactor as defined in claim 1 wherein said pipe in said intermediate section has an open end, and further comprising a grid covering said open end of said pipe in said intermediate section.

6. A reactor as defined in claim 1 wherein said agitating means is an axial-flow pump for directing the nutrient medium in axial direction and including an impeller, said extension pipe having a lower end provided with a collar which surrounds said impeller.

7. A reactor as defined in claim 6, and further comprising a flared transition conically widening toward said collar for connecting said extension pipe with said collar.

8. A reactor as defined in claim 6 wherein said collar defines a central axis, said impeller extending approximately in said axis.

9. A reactor as defined in claim 6, and further comprising baffles accommodated within said collar for preventing a rotation of reactor liquid during rotation of said impeller.

10. A reactor as defined in claim 6 wherein said interior space of said base has an inner wall surface, and further comprising baffles arranged in a space between said collar and said inner wall surface for deflecting reactor liquid vertically upwards to flow evenly through said support member.

11. A reactor as defined in claim 6, and further comprising gas supply means provided in said base and communicating with said circulation pipe above said agitating means.

12. A reactor as defined in claim 7, and further comprising gas supply means provided in said base and communicating with said circulation pipe above said agitating means, said gas supply means being connected to said flared transition.

13. A reactor as defined in claim 6 wherein said impeller includes blades adapted for reducing shear.

14. A reactor as defined in claim 6 wherein said agitating means includes a motor for allowing rotation of said impeller in both directions.