Indicator reagents, diagnostic assays and test kits employing organic polymer latex particles

• US 5,252,459 A
• Filed: 09/23/1988
• Issued: 10/12/1993
• Est. Priority Date: 09/23/1988
• Status: Expired due to Fees

• Current Assignee: Abbott Laboratories Incorporated
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Tarcha, Peter J., Wong, Martin, Donovan, James J.
• Primary Examiner(s): Johnston, Jill A.
• Application Number:
  US07/248,858
• Time in Patent Office: 1,845 Days
• Field of Search: 435/6, 435/7.4, 435/975, 435/7.7, 435/7.71, 435/7.9, 422/61, 422/55, 424/78, 436/501, 436/63, 436/518, 436/531, 436/548, 436/528, 436/534, 436/807, 436/533, 436/808
• US Class Current: 435/6.14
• CPC Class Codes: G01N 33/585 with a particulate label, e.g. coloured latex
• CPC Class Codes: Y10S 435/975 Kit
• CPC Class Codes: Y10S 436/808 Automated or kit

Abstract

An indicator reagent, assay method and test kit for determining the presence or amount of an analyte in a test sample, in which the indicator reagent is formed by attaching an organic polymer latex particle, preferably a colored particle, to a specific binding member. The specific binding member can be adsorbed onto or covalently bound to the organic polymer latex particles. The organic polymer latex particles are readily detected by direct visual observation or can be detected and/or measured by appropriate instrumentation.

Claims

1. An indicator reagent, useful for determining the presence or amount of an analyte in a test sample, comprising:

a. an organic polymer latex particle prepared from the polymerization of a plurality of nonchromophoric monomers, said particle selected from the group consisting of poly(pyrrole), polyphenylene, poly(aniline), poly(thiophene), poly(naphthalene), poly(thiophenol), polyacetylene and derivatives thereof, said particle having light absorbance characteristics resulting from a conjugated structure from the polymerization of said monomers wherein said polymer latex particle exhibits increased absorbance in the visible spectrum compared to the absorbance in the visible spectrum of the aggregate of nonchromophoric monomers from which it is prepared, and

b. a specific binding member directly or indirectly attached to said particle.

2. The indicator reagent according to claim 1, wherein said organic polymer latex particle is a colored particle.

3. The indicator reagent according to claim 1, wherein said organic polymer latex particle is a composite particle further comprising a polymer material selected from the group consisting of poly(vinyl chloride), polystyrene, poly(vinyl toluene), poly(acrylamide), poly(N-vinyl pyrrolidone) and derivatives thereof.

4. The indicator reagent according to claim 1, wherein said organic polymer latex particle is a copolymer comprising at least two different monomers.

5. The indicator reagent according to claim 4, wherein said monomers are selected from the group consisting of pyrrole, benzene, toluene, aniline, thiophene, naphthalene, thiophenol, acetylene and derivatives thereof.

6. The indicator reagent according to claim 1, wherein the specific binding member is selected from the group consisting of biotin, avidin, carbohydrates, lectins, complementary nucleic acid sequences, non-immunoreactant receptor molecules, complementary members to the non-immunoreactant receptor molecules, enzyme cofactors, enzymes, enzyme inhibitors and immunoreactants.

7. The indicator reagent according to claim 6, wherein said immunoreactant is selected from the group consisting of monoclonal antibody, polyclonal antibody, antibody fragment, recombinant antibody, recombinant protein, hapten, antigen and complexes thereof.

8. The indicator reagent according to claim 1, wherein said specific binding member is attached to said organic polymer latex particle by adsorption.

9. The indicator reagent according to claim 1, wherein said specific binding member is attached to said organic polymer latex particle by a linking group.

10. The indicator reagent according to claim 9, wherein said linking group is selected from the group consisting of carboxylic, amino, aldehyde, bromoacetyl, iodoacetyl, thiol, and epoxy reactive groups, residual free radicals and radical cations.

11. The indicator reagent according to claim 1, wherein said specific binding member of said indicator reagent is specific for a substance selected from the group consisting of the analyte as in a sandwich assay, a capture reagent as in a competitive assay and an ancillary specific binding member as in an indirect assay.

12. The indicator reagent according to claim 1, wherein said organic polymer latex particle becomes a colored particle upon further reaction with at least one additional nonchromophoric substance.

13. The indicator reagent according to claim 1, wherein said organic polymer latex particle is detectable by electron paramagnetic resonance.

14. A method for determining the presence or amount of an analyte in a test sample, comprising:

a. contacting the test sample sequentially or simultaneously with an indicator reagent and a capture reagent, directly or indirectly attached to a specific binding member, said indicator reagent comprising an organic polymer latex particle prepared from the polymerization of a plurality of nonchromophoric monomers, said particle having light absorbance characteristics resulting from a conjugated structure from the polymerization of said monomers wherein said polymer latex particle exhibits increased absorbance in the visible spectrum compared to the absorbance in the visible spectrum of the aggregate of nonchromophoric monomers from which it is prepared; and

b. allowing said indicator reagent to bind to the analyte in the test sample or to said capture reagent;

c. detecting said indicator reagent; and

d. determining thereby the presence or amount of analyte in the test sample.

15. The method according to claim 14, wherein said organic polymer latex particle is a colored particle.

16. The method according to claim 15, wherein said organic polymer latex particle is selected from the group consisting of poly(pyrrole), polyphenylene, poly(aniline), poly(thiophene), poly(naphthalene), poly(thiophenol), polyacetylene and derivatives thereof.

17. The method according to claim 14, wherein said organic polymer latex particle is a composite particle further comprising a polymer material selected from the group consisting of poly(vinyl chloride), polystyrene, poly(vinyl toluene), poly(acrylamide), poly(N-vinyl pyrrolidone) and derivatives thereof.

18. The method according to claim 14, wherein said organic polymer latex particle is a copolymer comprising at least two different monomers.

19. The method according to claim 18, wherein said monomers are selected from the group consisting of pyrrole, benzene, toluene, aniline, thiophene, naphthalene, thiophenol, acetylene and derivatives thereof.

20. The method according to claim 14, wherein said organic polymer latex particle comprises a particle which is coated with an organic polymer latex selected from the group consisting of poly(pyrrole), polyacetylene, polyphenylene, poly(aniline), poly(thiophene), poly(naphthalene), poly(thiophenol) and derivatives thereof.

21. The indicator reagent according to claim 14, wherein the specific binding member is selected from the group consisting of biotin, avidin, carbohydrates, lectins, complementary nucleic acid sequences, non-immunoreactant receptor molecules, complementary members to the non-immunoreactant receptor molecules, enzyme cofactors, enzymes, enzyme inhibitors and immunoreactants.

22. The method according to claim 21, wherein said immunoreactant is selected from the group consisting of monoclonal antibody, polyclonal antibody, antibody fragment, recombinant antibody, recombinant protein, hapten, antigen and complexes thereof.

23. The method according to claim 14, wherein said specific binding member is attached to said organic polymer latex particle by adsorption.

24. The method according to claim 14, wherein said specific binding member is attached to said organic polymer latex particle by a linking group.

25. The method according to claim 24, wherein said linking group is selected from the group consisting of carboxylic, amino, aldehyde, bromoacetyl, iodoacetyl, thiol, and epoxy reactive groups, residual free radicals and radical cations.

26. The method according to claim 14, wherein said specific binding member of said indicator reagent is specific for a substance selected from the group consisting of the analyte as in a sandwich assay, a capture reagent as in a competitive assay and an ancillary specific binding member as in an indirect assay.

27. The method according to claim 14, wherein said organic polymer latex particle becomes a colored particle upon further reaction with at least one additional nonchromophoric substance.

28. The method according to claim 14, wherein said organic polymer latex particle is detectable by electron paramagnetic resonance.

29. The indicator reagent according to claim 14, wherein said capture reagent is selected from the group consisting of biotin, avidin, carbohydrates, lectins, complementary nucleic acid sequences, non-immunoreactant receptor molecules, complementary members to the non-immunoreactant receptor molecules, enzyme cofactors, enzymes, enzyme inhibitors and immunoreactants.

30. The method according to claim 29, wherein said immunoreactant is selected from the group consisting of monoclonal antibody, polyclonal antibody, antibody fragment, recombinant antibody, recombinant protein, hapten, antigen and complexes thereof.

31. The method according to claim 29, wherein said specific binding member of said capture reagent is specific for a substance selected from the group consisting of the analyte as in a sandwich assay, said indicator reagent as in a competitive assay and an ancillary specific binding member as in an indirect assay.

32. The method according to claim 29, wherein said capture reagent is attached to a solid phase material.

33. The method according to claim 32, wherein said solid phase material is selected from the group consisting of magnetic particles, polystyrene beads, microtitre plates, nitrocellulose, polystyrene tubes, glass tubes, agarose, polyacrylamide beads and silicon particles.

34. The method according to claim 32, wherein said solid phase material is selected from the group consisting of microparticles and protein binding membranes.

35. The method according to claim 32, wherein said solid phase material is a porous, bibulous, absorbent or capillary material.

36. The method according to claim 29, wherein said capture reagent is attached to a plurality of microparticles, and wherein said microparticles are retained by a solid phase base material.

37. The method according to claim 29, wherein said capture reagent is attached to a carrier material.

38. The method according to claim 37, wherein said carrier material is bovine serum albumin.

39. The method according to claim 29, wherein at least one of said capture reagent and said indicator reagent is specific for at least one ancillary specific binding member.

40. A test kit for determining the presence or amount of an analyte in a test sample, comprising:

a. an indicator reagent comprising an organic polymer latex particle prepared from the polymerization of a plurality of nonchromophoric monomers, said particle selected from the group consisting of poly(pyrrole), polyphenylene, poly(aniline), poly(thiophene), poly(naphthalene), poly(thiophenol) polyacetylene and derivatives thereof, and having light absorbance characteristics resulting from a conjugated structure from the polymerization of said monomers wherein said polymer latex particle exhibits increased absorbance in the visible spectrum compared to the absorbance in the visible spectrum of the aggregate of nonchromophoric monomers from which it is prepared, said indicator reagent being directly or indirectly attached to a specific binding member and capable of producing a detectable signal indicative of the presence or amount of an analyte in a test sample; and

b. one or more assay components including capture reagents, ancillary binding members, solid phase material, buffers, water, stabilizers, surfactants, standards and calibrators.