Fiber optic sensors, apparatus, and detection methods using controlled release polymers and reagent formulations held within a polymeric reaction matrix

• US 5,252,494 A
• Filed: 03/30/1992
• Issued: 10/12/1993
• Est. Priority Date: 06/25/1986
• Status: Expired due to Fees

• Current Assignee: Trustees of Tufts College
• Original Assignee: Trustees of Tufts College
• Inventors: Walt, David R.
• Primary Examiner(s): Saunders, David
• Assistant Examiner(s): Chin, Christopher L.
• Application Number:
  US07/859,869
• Time in Patent Office: 561 Days
• Field of Search: 422/58, 422/82.05, 422/82.06, 422/82.07, 422/82.08, 422/82.09, 422/82.11, 435/808, 435/7.7, 435/7.72, 435/7.9, 435/288, 435/291, 436/164, 436/528, 436/531, 436/535, 436/805, 436/800, 436/807, 436/172, 385/123, 385/125, 385/144, 385/145, 356/73.1
• US Class Current: 436/528
• CPC Class Codes: G01N 2021/6434 Optrodes
• CPC Class Codes: G01N 2021/6484 Optical fibres
• CPC Class Codes: G01N 2021/772 Tip coated light guide
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 2021/7793 Sensor comprising plural indicators
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 436/80 Fluorescent dyes, e.g. rhodamine
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/807 Apparatus included in process claim, e.g. physical support structures

Abstract

An improved fiber optic sensor, sensing apparatus, and methods for making optical detections are provided. The fiber optic sensor employs a fiber optic strand to convey light energy, an immobilized polymeric reaction matrix, and at least one controlled release polymeric carrier within said reaction matrix comprising a controlled release polymer material and a releasable reagent formulation able to react with the analyte of interest. The optic sensors and sensor construction have been demonstrated to be both functionally useful and long serving in duration.

Claims

1. A fiber optic sensor for performing optical determinations of a plurality of test fluids believed to contain an analyte of interest, said fiber optic sensor comprising:

a optical fiber strand able to convey light energy of a determinable wavelength, said optical fiber strand having a proximal end, a distal end, and a strand length;

a discrete, porous polymeric reaction matrix immobilized at the distal end of said optical fiber strand, said analyte of interest to be detected being able to permeate and react within said polymeric reaction matrix; and

at least one preformed controlled release polymer carrier contained within said immobilized porous polymeric reaction matrix, each of said preformed controlled release polymer carrier comprising at least one controlled release polymer material and at least one reagent formation controllably releasable from said polymer material in mobile form for reaction with such analyte of interest as is present within said polymeric reaction matrix, the reaction between said analyte of interest and said released reagent formulation forming a diffusible and optically detectable product within said polymeric reaction matrix.

2. The fiber optic sensor as recited in claim 1 wherein said reagent formulation is selected from the group consisting of indicator compounds and dyes.

3. The fiber optic sensor as recited in claim 1 wherein said reagent formulation is selected from the group consisting of labelled antigens, labelled haptens and labelled antibodies and antibody fragments.

4. The fiber optic sensor as recited in claim 1 wherein said reagent formulation is selected from the group consisting of enzymes and zymogens, enzyme substrates and substrate analogues, and cofactors.

5. The fiber optic sensor as recited in claim 1 wherein said controlled release polymer is a polyanhydride.

6. The fiber optic sensor as recited in claim 1 wherein said controlled release polymer is a vinyl polymer.

7. The fiber optic sensor as recited in claim 1 wherein said polymeric reaction matrix is a polyacrylamide.

8. The fiber optic sensor as recited in claim 1 wherein said controlled release polymer carrier is fluid erodible.

9. A fiber optic sensing apparatus for performing optical determinations of a plurality of test fluids believed to contain an analyte of interest, said sensing apparatus comprising:

a fiber optic sensor comprised of;

a optical fiber strand able to convey light energy of a determinable wavelength, said optical fiber strand having a proximal end, a distal end, and a strand length,

a discrete, porous polymeric reaction matrix immobilized at the distal end of said optical fiber strand, said analyte of interest to be detected being able to permeate and react within said polymeric reaction matrix, and

at least one preformed, controlled release polymer carrier contained within said immobilized porous polymeric reaction matrix, each of said preformed controlled release polymer carrier comprising at least one controlled release polymer material and at least one reagent formulation controllably releasable from said polymer material in mobile form for reaction with such analyte of interest as is present within said polymeric reaction matrix, the reaction between said analyte of interest and said released reagent formulation forming a diffusible and optically detectable product within said polymeric reaction matrix;

a source of light energy of determinable wavelength;

means for conveying light energy from said source to said proximal end of said optical fiber strand;

means for detecting light energy; and

means for conveying light energy from said optical fiber strand to said detecting means.

10. The fiber optic sensing apparatus as recited in claim 9 further comprising a computer control and data acquisition system.

11. The fiber optic sensing apparatus as recited in claim 9 wherein said light energy is in the ultraviolet light range.

12. The fiber optic sensing apparatus as recited in claim 9 wherein said light energy is in the visible light range.

13. A method for performing optical detections of a plurality of test fluids believed to contain an analyte of interest, said method comprising the steps of:

providing a fiber optic sensor comprised of;

a optical fiber strand able to convey light energy of a determinable wavelength, said optical fiber strand having a proximal end, a distal end, and a strand length,

a discrete, porous polymeric reaction matrix immobilized at the distal end of said optical fiber strand, said analyte of interest to be detected being able to permeate and react within said polymeric reaction matrix, and

at least one preformed, controlled release polymer carrier contained within said immobilized porous polymeric reaction matrix, each of said preformed controlled release polymer carrier comprising at least one controlled release polymer material and at least one reagent formulation controllably releasable from said polymer material in mobile form for reaction with such analyte of interest as is present within said polymeric reaction matrix, the reaction between said analyte of interest and said release reagent formulation forming a diffusible and optically detectable product within said polymeric reaction matrix;

introducing a test fluid to the distal end of said fiber optical sensor whereby at least a portion of such analyte of interest as is present in said fluid permeates said polymeric reaction matrix and reacts with such reagent formulation as has been controllably release into said polymeric reaction matrix to form a diffusible and optically detectable product;

conveying light energy of determinable wavelength to said proximal end of said optical fiber strand; and

detecting light energy conveyed by said optical fiber strand from said polymeric reaction matrix after formation of said diffusible and optically detectable reaction product by said release reagent formulation and the analyte of interest, said detected light energy being a measure of the analyte of interest in the test fluid.