Fluorescent compound, complex, reagent, and specific binding assay employing said reagent
First Claim
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1. A fluorescent compound expressed by formula A:
- ##STR11## wherein m represents 1 or 2, n represents an integer selected from the group consisting of 0, 1, 2, 3 and 4;
R1 is independently selected from the group consisting of hydrogen atom, an alkyl group, an alkenyl group, an alkynyl group, an aralkyl group, and an aryl group having carbon atoms up to 14;
R2 is independently selected from the group consisting of hydrogen atom, an aryl group having carbon atoms up to 14, and an alkyl group;
R3 is a functional group represented by --R3-1 --R3-2, wherein R3-1, although its presence is not indispensable, is independently selected from the group consisting of an alkylene group, an arylene group having carbon atoms up to 14, and an aralkylene group, nd R3-2 is indispensable and independently selected from the group consisting of hydrogen atom, an alkyl group, an aryl group having carbon atoms up to 14, carboxyl group, hydroxyl group, an alkoxyl group, an amino group, an amide group, sulfonamide group, sulfide group, sulfoxide group, sulfone group, a halogen atom, carbonyl group, and nitro group; and
R4 is independently selected from the group consisting of hydrogen atom, an alkyl group, an alkenyl group, an alkynyl group, an aryl group having carbon atoms up to 14, carboxyl group, hydroxyl group, an alkoxyl group, an amino group, an amide group, sulfonamide group, sulfide group, sulfoxide group, sulfone group, nitro group, a halogen atom, mercapto group, carbonyl group, and a functional group represented by --R4-1 --R4-2, wherein R4-1, although its presence is not indispensable, is independently selected from the group consisting of an alkylene group, an alkenylene group, an arylene group having carbon atoms up to 14, and an aralkylene group, and R4-2 is indispensable and independently selected from functional groups;
##STR12## wherein X is selected from the group consisting of a halogen atom, --OSO3 CH3, --OSO2 F, --OSO2 CF3, --SO2 C4 F9, and ##STR13## R is selected from the group consisting of an alkyl group, an alkenyl group, an aryl group having carbon atoms up to 14, and aralkyl group, RB is selected from the group consisting of an alkylene group, an alkenylene group, an arylene group having carbon atoms up to 14, and an aralkylene group, and 1 represents an integer of from 0 to 5, p represents an integer of from 2 to 10; and
wherein, R4 may optionally take a form of either at least one aromatic ring or at least one heterocyclic ring having N as heteroatom and aromatic 5 or 6 membered ring or fused ring thereof, when the heterocyclic ring has two or more N atoms, they are separated by one or two carbon atoms, wherein the heterocyclic ring includes two or three carbon atoms, which are the part of a phenanthroline ring as a result of the ring-forming condensation brought about by the bonding of neighboring R4 to each other.
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Abstract
A fluorescent compound, which is readily capable of forming a stable complex with a rare-earth metal ion having fluorescence with satisfactory intensity even in an aqueous system and long fluorescence lifetime when formed a complex with the rare-earth metal ion, a complex of the fluorescent compound and the rare-earth metal ion, and a labelled reagent made therefrom, together with a specific binding assay using said labelling agent.
A fluorescent compound expressed by the following formula A, typically exemplified by 2,15-diaza[3,3](2,9)-1,10-phenanthrolinophane-N2,N15 -diacetic acid. ##STR1##
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13 Claims
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1. A fluorescent compound expressed by formula A:
- ##STR11## wherein m represents 1 or 2, n represents an integer selected from the group consisting of 0, 1, 2, 3 and 4;
R1 is independently selected from the group consisting of hydrogen atom, an alkyl group, an alkenyl group, an alkynyl group, an aralkyl group, and an aryl group having carbon atoms up to 14; R2 is independently selected from the group consisting of hydrogen atom, an aryl group having carbon atoms up to 14, and an alkyl group; R3 is a functional group represented by --R3-1 --R3-2, wherein R3-1, although its presence is not indispensable, is independently selected from the group consisting of an alkylene group, an arylene group having carbon atoms up to 14, and an aralkylene group, nd R3-2 is indispensable and independently selected from the group consisting of hydrogen atom, an alkyl group, an aryl group having carbon atoms up to 14, carboxyl group, hydroxyl group, an alkoxyl group, an amino group, an amide group, sulfonamide group, sulfide group, sulfoxide group, sulfone group, a halogen atom, carbonyl group, and nitro group; and R4 is independently selected from the group consisting of hydrogen atom, an alkyl group, an alkenyl group, an alkynyl group, an aryl group having carbon atoms up to 14, carboxyl group, hydroxyl group, an alkoxyl group, an amino group, an amide group, sulfonamide group, sulfide group, sulfoxide group, sulfone group, nitro group, a halogen atom, mercapto group, carbonyl group, and a functional group represented by --R4-1 --R4-2, wherein R4-1, although its presence is not indispensable, is independently selected from the group consisting of an alkylene group, an alkenylene group, an arylene group having carbon atoms up to 14, and an aralkylene group, and R4-2 is indispensable and independently selected from functional groups;
##STR12## wherein X is selected from the group consisting of a halogen atom, --OSO3 CH3, --OSO2 F, --OSO2 CF3, --SO2 C4 F9, and ##STR13## R is selected from the group consisting of an alkyl group, an alkenyl group, an aryl group having carbon atoms up to 14, and aralkyl group, RB is selected from the group consisting of an alkylene group, an alkenylene group, an arylene group having carbon atoms up to 14, and an aralkylene group, and 1 represents an integer of from 0 to 5, p represents an integer of from 2 to 10; andwherein, R4 may optionally take a form of either at least one aromatic ring or at least one heterocyclic ring having N as heteroatom and aromatic 5 or 6 membered ring or fused ring thereof, when the heterocyclic ring has two or more N atoms, they are separated by one or two carbon atoms, wherein the heterocyclic ring includes two or three carbon atoms, which are the part of a phenanthroline ring as a result of the ring-forming condensation brought about by the bonding of neighboring R4 to each other. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
- ##STR11## wherein m represents 1 or 2, n represents an integer selected from the group consisting of 0, 1, 2, 3 and 4;
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9. The compound 2,15-diaza[3,3](2,9)-1,10-phenanthrolinophane-N2,N15 -diacetic acid.
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10. The compound 8-(3'"'"'-succinimidyloxycarbonylpropionamide-2,15-diaza[3,3](2,9)-1,10-phenanthrolinophane-N2,N15 -diacetic acid.
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11. The compound 7,10-diphenyl-2,15-diaza[3,3](2,9)-1,10-phenanthrolinophane-N2,N.sup.15 -diacetic acid.
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12. The compound 7,10-bis(chlorosulfophenyl)-2,15-diaza(3,3](2,9)-1,10-phenanthrolinophane-N2,N15 -diacetic acid.
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13. The compound 7,10,20,23-tetrakis(chlorosulfophenyl)-2,15-diaza[3,3](2,9)-1,10-phenanthrolinophane-N2,N15 -diacetic acid.
Specification