Method for the modification of the expression characteristics of an endogenous gene of a given cell line

  • US 5,272,071 A
  • Filed: 05/28/1992
  • Issued: 12/21/1993
  • Est. Priority Date: 12/22/1989
  • Status: Expired due to Term
First Claim
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1. A method of activating a predetermined normally transcriptionally silent gene within the genome of a cell line so as to enable said cell line to express the gene product of said gene, comprising inserting a DNA construct into said genome by homologous recombination, said DNA construct comprising a DNA regulatory segment capable of stimulating expression of said gene when operatively linked thereto and a DNA targeting segment homologous to a region of said genome within or proximal to said gene, wherein said construct is inserted such that said regulatory segment is operatively linked to said gene of interest.

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