Multiwell stat test

• US 5,288,648 A
• Filed: 07/24/1992
• Issued: 02/22/1994
• Est. Priority Date: 12/01/1989
• Status: Expired due to Fees

• Current Assignee: SangStat Medical Corporation (Sanofi )
• Original Assignee: SangStat Medical Corporation (Sanofi )
• Inventors: Pouletty, Philippe, Atwood, Beth, Rammler, David
• Primary Examiner(s): WARDEN, JILL ALICE
• Application Number:
  US07/919,295
• Time in Patent Office: 578 Days
• Field of Search: 422/56-58, 422/101, 422/61, 436/810, 436/530, 436/514, 436/518
• US Class Current: 436/514
• CPC Class Codes: C23C 16/487 using electron radiation
• CPC Class Codes: G01N 33/5302 Apparatus specially adapted for immunological test procedures
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: Y10S 435/971 Capture of complex after antigen-antibody reaction
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick

Abstract

An apparatus is provided for the detection and semi-quantitative measurement of analytes. The assay results are visualized by the formation on a filter of a colored annular or circular spot, the diameter of the spot being related to the concentration of the analyte of interest. The filter on which the assay results are visualized is divided into multiple regions by strips of non-porous tape crossing the filter surface. The invention also includes a component for diluting sample to a suitable concentration for analysis, and dispensing the diluted sample onto the test filter.

Claims

1. A diagnostic device for measuring an analyte which is a member of a specific binding pair, where a label is employed for said measuring which label provides for a visual detectable signal, said device comprising:

a housing comprising an upper reservoir portion and a lower casing portion for holding at least one layer;

in said casing in the direction of flow;

a porous reactive filter as the floor of said reservoir component comprising a measurement circle of a member of a specific binding pair immobilized to said filter and defining a concentration gradient comprising an inner circle at an elevated concentration and an outer contiguous circle at a substantially lower concentration; and

fluid receiving means for receiving fluid from said porous reactive filter.

2. A device according to claim 1, wherein said fluid receiving means comprises:

a porous separation layer;

a flowrate control layer for reducing the rate of flow; and

an absorbent waste fluid receiving layer.

3. A device according to claim 1, wherein said porous reactive filter is divided into a plurality of regions separated by non-porous dividers, wherein at least two of said regions comprise at least one said measurement circle.

4. A device according to claim 3, wherein at least one of said regions comprises two said measurement circles.

5. A device according to claim 1, wherein said housing is a molded plastic housing.

6. A device according to claim 1, wherein the average concentration of said inner circle is at least two fold greater than the highest concentration in the outer circle.

7. A diagnostic device for measuring an analyte comprising a porous reactive filter comprising at least one region and in at least one of said regions, a measurement circle of a member of a specific binding pair immobilized to said filter and defining a concentration gradient comprising an inner circle at an elevated concentration and an outer contiguous circle at a substantially lower concentration.

8. A diagnostic device according to claim 7, wherein said device comprises at least two regions separated by a non-porous divider.

9. A diagnostic device according to claim 7, wherein at least one said region comprises at least two measurement circles having the same specific binding pair member.

10. A diagnostic device according to claim 7, wherein said porous reactive filter is filter paper.