Speciman support for optical analysis

• US 5,290,705 A
• Filed: 01/13/1992
• Issued: 03/01/1994
• Est. Priority Date: 01/13/1992
• Status: Expired due to Fees

• Current Assignee: R.E. Davis Chemical Corporation
• Original Assignee: R.E. Davis Chemical Corporation
• Inventors: Davis, Robert E.
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): TRAN, LIEN THUY
• Application Number:
  US07/820,302
• Time in Patent Office: 778 Days
• Field of Search: 422/57, 422/102, 422/99, 422/104, 356/244, 356/323, 356/246, 436/164, 446/15, 446/21, D21/61
• US Class Current: 436/164
• CPC Class Codes: B01L 2300/0822 Slides
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 3/5088 confining liquids at a location by surface tension, e.g. virtual wells on plates, wires B01L3/50857 takes precedence
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 21/03 Cuvette constructions
• CPC Class Codes: G01N 21/35 using infrared light G01N21/39 takes precedence
• CPC Class Codes: G01N 21/3577 for analysing liquids, e.g. polluted water

Abstract

A specimen support for optical observation or analysis. The support comprises a disc-like member composed of a rigid material and having at least one unobstructed hole extending therethrough. When analyzing a liquid specimen, the disc is dipped in the liquid and the surface tension of the liquid will cause the liquid to bridge or span the hole. The support is then placed in a tray or holder and exposed to an infrared beam to generate an infrared spectrum.

Claims

1. A method of analyzing a liquid specimen, comprising the steps of forming a specimen support of a rigid material that is non-reactive to water and acidic substances and having a pair of opposed surfaces and having a plurality of unobstructed holes extending between said surfaces, a first of said holes having a different cross sectional area than a second of said holes, positioning said support so that said surfaces are horizontal and the axes of the holes are vertical, enclosing each hole with an unsupported film of a liquid specimen to be analyzed, directed a beam of infrared light through the hole and through the liquid film enclosing the hole, and generating a wavelength spectrum of said beam passing through said film.

2. The method of claim 1, wherein the step of enclosing the holes comprises dipping the support in a quantity of said liquid specimen.

3. The method of claim 1, and including the step of forming the film of each liquid specimen with a thickness sufficient to provide a spectrum when said beam is passed through said film, said film having a pair of generally parallel surfaces substantially free of convexity that extend transversely across said holes.

4. The method of claim 1, and including the step of maintaining the thickness of said film at a value less than that necessary to cause lensing.

5. An analytic specimen support for infrared microspectroscopy, comprising a holder having a confined opening extending therethrough, support means provided in the opening, and a specimen support removably disposed in the opening and mounted on said support means, said specimen support composed of a rigid material that is non-reactive to water, acidic substances and solvents, said specimen support including a pair of opposed generally flat horizontal surfaces and having a plurality of unobstructed holes extending between said surfaces with the axes of the holes being vertical, a film of a liquid specimen spanning each hole and held in said hole by the surface tension of the liquid, the film spanning each of said holes having generally parallel opposed surfaces and being free of convexity, a first of said holes having a different cross sectional area than a second of said holes. 15

6. The support of claim 5, wherein said holes have a diameter in the range of 10 microns to 13 millimeters.