Fluorescent pH indicators

• US 5,302,731 A
• Filed: 07/13/1992
• Issued: 04/12/1994
• Est. Priority Date: 07/13/1992
• Status: Expired due to Fees

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Pitner, J. Bruce, Hoke, Randal A.
• Primary Examiner(s): Chan, Nicky
• Application Number:
  US07/912,426
• Time in Patent Office: 638 Days
• Field of Search: 549/227, 549/394
• US Class Current: 549/394
• CPC Class Codes: C07D 311/82 Xanthenes
• CPC Class Codes: C09B 11/24 Phthaleins containing amino groups ; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes
• CPC Class Codes: G01N 31/221 for investigating pH value

Abstract

Fluorescent pH indicator compounds which exhibit an increase in fluorescence intensity with decreasing pH. The indicators are derivatives of rhodamine and sulforhodamine type dyes and are particularly useful for measuring pH in acidic environments such as carbon dioxide production by microorganisms and pH of certain acidic intracellular compartments.

Claims

1. A fluorescent pH indicator compound having the structure:

##STR4##

2. A fluorescent pH indicator compound having the structure:

##STR5##