Dispensing assembly with interchangeable cartridge pumps

• US 5,316,452 A
• Filed: 05/11/1992
• Issued: 05/31/1994
• Est. Priority Date: 05/11/1992
• Status: Expired due to Term

• Current Assignee: Dako Denmark A/S (Agilent Technologies, Inc.)
• Original Assignee: Gilbert Corporation
• Inventors: Bogen, Steven A., Loeffler, Herbert H.
• Primary Examiner(s): Bertsch, Richard A.
• Assistant Examiner(s): KORYTNYK, PETER G
• Application Number:
  US07/881,397
• Time in Patent Office: 750 Days
• Field of Search: 417/412, 417/474, 417/478, 417/479, 222/181, 222/207, 222/214, 128/DIG. 12, 604/153, 604/151
• US Class Current: 417/412
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 3/0293 for liquids
• CPC Class Codes: B67D 7/0216 by squeezing collapsible or flexible storage containers B67D7/0255 takes precedence; for dispensing beverages on draught B67D1/0001
• CPC Class Codes: F04B 43/08 having tubular flexible members F04B43/12 takes precedence
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: G01N 35/1002 Reagent dispensers

Abstract

This invention describes a cartridge pump and dispensing assembly for applications where cartridges containing liquid reagents are interchanged often. The cartridge pump comprises a reagent reservoir which directly empties into a metering chamber. A valve is at each end of the metering chamber. The two valves are aligned in the same direction so as to allow unidirectional liquid flow. The metering chamber is made of a compressible material, such as flexible tubing, so that when an external compression is applied to the chamber, the liquid contained therein is forcibly expelled. As the compression is removed, the metering chamber resumes its former shape and draws liquid into the chamber from the reagent reservoir. A dispensing assembly with electromechanical actuators for compression of the metering chamber and a means for sensing the amount of liquid contained within the reagent reservoir are also shown.

Claims

1. A dispensing assembly comprising:

A) a plurality of pump cartridges, each pump cartridge comprising;

i) a reservoir for containing a liquid, said reservoir having a liquid flow outlet,

ii) a metering chamber extending below the reservoir, said metering chamber directly connected to said liquid flow outlet, said metering chamber comprising a compressible housing having an noncompressed shape, and

iii) a one-way inlet valve and a one-way outlet valve at each end of said compressible housing, said valves aligned in the same direction so as to allow unidirectional fluid flow from the reservoir;

B) an electrically driven reciprocating hammer actuator capable of compressing said compressible housing of each pump cartridge to eject a volume of liquid from the metering chamber thorough the outlet valve, and then release the housing to return the housing to the non-compressed shape to draw a volume of liquid with the metering chamber through the inlet valve;

C) a pump cartridge frame with a plurality of receptacles into which said plurality of pump cartridges are fit for holding each pump cartridge in a fixed position with respect to said actuator; and

D) a second frame supporting the actuator and removably receiving the pump cartridge frame with the plurality of receptacles as a unit to position each pump cartridge adjacent to the actuator, the first frame being removable from the second frame by lifting the first frame to lift all of the plurality of pump cartridges away from the second frame and the actuator.

2. A dispensing assembly according to claim 1 wherein said reservoir contains a plunger above said liquid in said reagent reservoir, said plunger being capable of moving within said reservoir as liquid is drawn out of said reservoir through said liquid flow outlet.

3. A dispensing assembly according to claim 2 wherein said plunger has a frictional force against the inner wall of said reservoir greater than said gravity pressure of said liquid in said reservoir.

4. A dispensing assembly according to claim 1 wherein said reservoir has a one-way valve at the top of said reservoir.

5. A dispensing assembly according to claim 1 wherein said reservoir has a rolling diaphragm at the top of said reservoir.

6. A dispensing assembly according to claim 1 wherein said reservoir has a small aperture at the top of said reservoir.

7. A dispensing assembly according to claim 1 wherein each pump cartridge has on its external surface one or more ridges projecting outward from the external surface of said cartridge serving as keys.

8. A dispensing assembly according to claim 1 with a means for reducing the flow velocity of said liquid during said ejection comprising a nozzle with an inner diameter which is greater than the opening of said outlet valve.

9. A dispensing assembly according to claim 1 wherein said outlet valve is normally closed and has an opening pressure greater than the gravity pressure applied by said liquid in said reservoir.

10. A dispensing assembly according to claim 1, wherein said cartridge has on its external surface one or more ridges projecting outward from the external surface of said cartridge serving as keys and said frame has a means for accommodating said ridges of said pump cartridge.

11. A dispensing assembly according to claim 1 which further includes:

a plurality of electromechanical actuators.

12. A dispensing assembly according to claim 1 which further includes a compressible piston hammer mounted on a piston arm of said actuator.

13. A dispensing assembly according to claim 1 wherein the reservoir contains biological reagent.

14. A dispensing assembly as claimed in claim 1 wherein the second frame is a moveable platform.

15. A pump cartridge assembly for dispensing liquid from a plurality of pump cartridges comprising:

A) a pump cartridge frame with a plurality of receptacles into which a plurality of pump cartridges are fit, each pump cartridge comprising;

i) a reagent reservoir for containing liquid, said reservoir having a liquid flow outlet,

ii) a metering chamber extending below the reservoir, said metering chamber being directly connected to said liquid flow outlet, said metering chamber comprising a compressible housing having a non-compressed shape, and

iii) a one-way inlet valve and a one-way outlet valve at each end of said compressible housing, said valves aligned in the same direction so as to allow unidirectional fluid flow from the reservoir; and

B) the pump cartridge frame and the plurality of pump cartridges being removable as a unit from an actuator assembly, the actuator assembly having an electrically driven reciprocating hammer for compressing each compressible housing for the unidirectional ejection of a volume of liquid from said metering chamber and the pump cartridge, said compressible housing returning to said noncompressible shape after cessation of compression by the hammer to draw an additional volume of liquid into said metering chamber, the pump cartridge frame being removable from the actuator assembly by lifting the pump cartridge frame to lift all of the plurality of pump cartridges away from the actuator assembly, removal of the pump cartridges removing all dispensing surfaces wetted by fluid from said reagent reservoir.

16. A method of dispensing liquid comprising:

A) providing an actuator assembly which includes an electrically driven reciprocating hammer;

B) positioning as a unit a plurality of pump cartridges, on a pump cartridge frame, within the actuator assembly, each pump cartridge comprising;

i) a reagent reservoir for containing a liquid, said reservoir having a liquid flow outlet,

ii) a metering chamber extending below the reservoir, said metering chamber being directly connected to said liquid flow outlet, said metering chamber comprising a compressible housing having an non-compressed shape, and

iii) a one-way inlet valve and a one-way outlet valve at each end of said compressible housing, said valves aligned in the same direction so as to allow unidirectional fluid flow from the reservoir;

C) compressing the compressible housing with the reciprocating hammer of the actuator assembly to unidirectionally eject a volume of liquid from said metering chamber and the pump cartridge;

D) returning the reciprocating hammer to return the compressible housing to its noncompressed shape to draw an additional volume of liquid into said metering chamber; and

E) removing the pump cartridge frame and pump cartridges as a unit from the actuator assembly by lifting the pump cartridge frame to lift all of the plurality of pump cartridges away form the actuator assembly, removal of the pump cartridges providing for replacement of all dispensing surfaces wetting by fluid from said regent reservoirs.