Methods of using labeled nucleotides

CAFC
  • US 5,328,824 A
  • Filed: 12/08/1987
  • Issued: 07/12/1994
  • Est. Priority Date: 04/17/1981
  • Status: Expired due to Term
First Claim
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1. A method of detecting the presence or absence of a nucleic acid in a sample which comprises the steps of(a) contacting under hybridizable conditions said sample with at least one compound comprising the structure:

  • ##STR26## wherein each of B'"'"' and B" represents a purine, 7-deazapurine, or pyrimidine moiety covalently bonded to the C1'"'"' -position of the sugar moiety, provided that whenever B'"'"' or B" is purine or 7-deazapurine, the sugar moiety is attached at the N9 -position of the purine or 7-deazapurine, and whenever B'"'"' or B" is pyrimidine the sugar moiety is attached at the N1 -position of the pyrimidine;

    wherein B represents 7-deazapurine or pyrimidine moiety covalently bonded to the C1'"'"' -position of the sugar moiety, provided that whenever B is 7-deazapurine, the sugar moiety is attached at the N9 -position of the 7-deazapurine, and whenever B is pyrimidine the sugar moiety is attached at the N1 -position of the pyrimidine;

    wherein A comprises at least three carbon atoms and represents at least one component of a signalling moiety capable of producing a detectable signal;

    wherein B and A are covalently attached directly or indirectly through a linkage group, said linkage group not interfering substantially with the characteristic ability of said compound to hybridize with said nucleic acid or of A to be detected;

    wherein if B is 7-deazapurine, A is attached to the 7-position thereof, and if B is pyrimidine, A is attached to the 5-position thereof;

    wherein m, n and p are integers, provided that m and p are not simultaneously 0 and provided further n is never 0; and

    wherein z represents H-- or HO--; and

    (b) detecting said compound or compounds so as to detect said nucleic acid.

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