Method and apparatus for material analysis

• US 5,348,002 A
• Filed: 04/23/1992
• Issued: 09/20/1994
• Est. Priority Date: 04/23/1992
• Status: Expired due to Term

• Current Assignee: Sirraya Incorporated
• Original Assignee: Sirraya Incorporated
• Inventors: Caro, Richard G.
• Primary Examiner(s): Sykes, Angela D.
• Application Number:
  US07/872,926
• Time in Patent Office: 880 Days
• Field of Search: 128/633-635, 128/653.1, 128/653.4, 128/654, 128/664-667, 128/637, 356/39-41
• US Class Current: 600/310
• CPC Class Codes: A61B 5/0095 by applying light and detecting acoustic waves, i.e. photoacoustic measurements
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: A61B 5/14552 Details of sensors specially adapted therefor
• CPC Class Codes: A61B 5/6826 Finger
• CPC Class Codes: A61B 5/6838 Clamps or clips
• CPC Class Codes: G01N 2291/015 Attenuation, scattering
• CPC Class Codes: G01N 2291/02475 Tissue characterisation
• CPC Class Codes: G01N 2291/02483 Other human or animal parts, e.g. bones
• CPC Class Codes: G01N 2291/02809 Concentration of a compound, e.g. measured by a surface mass change
• CPC Class Codes: G01N 29/14 using acoustic emission techniques echo of particles G01N29/046; measuring mechanical vibrations or acoustic waves in solids in general G01H1/00
• CPC Class Codes: G01N 29/2412 using the magnetostrictive properties of the material to be examined, e.g. electromagnetic acoustic transducers [EMAT]; investigating the presence of flaws using eddy currents G01N27/90, magnetostrictive transducers B06B1/08, measuring magnetostrictive properties G01R33/18
• CPC Class Codes: G01N 29/449 Statistical methods not provided for in G01N29/4409, e.g. averaging, smoothing and interpolation

Abstract

Disclosed is a method and apparatus for determining the presence and/or concentration of chemical species which absorb electromagnetic energy, dependent to a degree upon the chemical species and the wavelength of electromagnetic energy applied to matter including said species. The absorbed electromagnetic energy generates acoustic energy which is detected and analyzed to determine the presence and/or concentration of the chemical species in the matter.

Claims

1. The method of determining the concentration of a chemical species in condensed that scatters and absorbs electromagnetic energy comprising the steps of

exciting a volume of said matter with electromagnetic energy of predetermined wavelength to cause said volume to generate acoustic energy,

detecting said acoustic energy and generating a signal representative of the amplitude of the acoustic energy;

developing a relationship between the amplitude of the acoustic energy generated in said condensed matter and the degree of absorption of said electromagnetic energy by said matter and chemical species; and

analyzing the amplitude of said acoustic energy, making use of said relationship to determine the concentration of said species.

2. The method as in claim 1 in which the condensed matter is a biological material.

5. The method as in claims 1, 3 or 4 in which the scattering coefficient μ

, of the sample and the sample thickness L are selected such that μ

_s L>

>

1.

6. The method of claim 1, 3 or 4 in which the analysis is by multivariate analysis.

7. The method of claims 1, 3 or 4 in which the analysis is performed by comparison of the measured signals for the selected wavelengths with signals obtained from known or partially known mixtures of chemical species in said sample material.

8. The method as in claims 1, 3 or 4 in which the chemical species is glucose in blood.

9. The method as in claims 1, 3 or 4 in which the chemical species is oxygen in blood.

11. The method as in claims 1, 3, or 4 in which the analysis is made by partial least square correlation.

3. The method of determining the concentration of multiple or single chemical species in a sample which both scatters and absorbs electromagnetic energy comprising the steps of

irradiating the sample with electromagnetic energy at selected wavelengths to cause the sample to generate acoustic energy;

detecting the acoustic energy and generating signals representative of the amplitude of said acoustic energy at said wavelengths;

developing a relationship between the amplitude of said acoustic energy in said sample and the degree of absorption by said sample and multiple or single species for each selected wavelength; and

analyzing the dependence of the amplitude of said acoustic signal on the selected wavelength of the electromagnetic energy, making use of said relationship to determine the concentration of said multiple or single species in said sample.

4. The method of claim 3 including the additional steps of

detecting the irradiating electromagnetic energy at said selected wavelengths after it has traversed a volume of said sample and generating intensity signals representative of the intensity of said electromagnetic radiation at said wavelengths;

analyzing the dependence of said intensity signals on the selected wavelengths of the electromagnetic energy to determine the concentration of said multiple or single species; and

correlating said electromagnetic and acoustic analysis to enhance the accuracy of said determination of concentration of said multiple or single species.

10. The method as in claims 3 or 4 in which the sample is biological material.

12. The method of determining the absorption coefficient of a biological substance comprising the steps of

exciting a volume of said biological substance with electromagnetic energy of predetermined wavelength to cause said volume to generate acoustic energy within said medium whose amplitude is dependent upon the wavelength and intensity of said electromagnetic energy and the absorption coefficient of said biological substance at said wavelength;

detecting said acoustic energy and generating a signal representative of the amplitude of said acoustic energy;

developing a relationship between the amplitude of said acoustic energy in said biological substance and the degree of absorption of said electromagnetic energy, from said relationship and from the amplitude of the acoustic signal, determining the absorption coefficient of said biological substance at said wavelength.

18. The method as in claim 12, 13, 14, 15, or 16 in which the acoustic energy is gated to detect acoustic signals generated at a selected volume.

24. The method as in claims 12, 13, 14, 15, 16, 20 or 17 including the additional step of detecting electromagnetic energy which is transmitted through the material to generate a transmitted radiation signal and comparing the intensity of the transmitted signal to the intensity of the irradiation signal to determine an optical absorption coefficient.

13. The method of determining the concentration of a component species in an electromagnetic energy scattering medium comprising the steps of

irradiating said medium with electromagnetic energy at selected wavelengths to cause said medium to generate acoustic energy within said medium whose amplitude is dependent upon the wavelength and intensity of said electromagnetic energy and the absorption coefficient of said component and scattering medium at said wavelength;

scanning the wavelength of said electromagnetic energy over a predetermined range of frequencies;

measuring the intensity of said electromagnetic energy at said wavelengths and generating an intensity signal representative of the intensity at each of said wavelengths;

detecting the acoustic energy at said wavelengths to provide an output signal having an amplitude representative of the amplitude of said acoustic energy at said wavelengths; and

determining the concentration of said component species by comparing the electromagnetic energy signal and the acoustic amplitude signal with known signals for component species in a scattering medium.

14. The method as in claim 13 in which the steps of irradiating, scanning, measuring and detecting are repeated a predetermined number of times and the electromagnetic energy intensity signals and the acoustic amplitude signals are stored and then averaged and the averaged signals are then used to determined the concentration of said species.

19. The method as in claims 14 or 16 wherein the component is oxygen.

22. The method as in claims 13, 14, 15, 16, 20 or 17 in which the component is within biological tissue.

23. The method as in claim 13, 14, 15, 16, 20, or 17 in which the component is in vitro material.

25. The method as in claims 13, 16 or 17 wherein the component is blood glucose.

15. The method of determining the concentration of a component in a biological material, said method comprising the steps of

exciting a volume of said material with electromagnetic energy to cause a finite volume of said material to generate acoustic energy whose amplitude is dependent upon the intensity and wavelength of said electromagnetic energy and the absorption coefficient of said component at said wavelength;

detecting said generated acoustic energy and generating a representative acoustic signal;

developing a relationship between the amplitude of said acoustic energy in said biological material and the degree of absorption of said electromagnetic energy by said biological material and said component;

determining by analysis from the intensity of the electromagnetic energy and the amplitude of the acoustic signal the absorption coefficient at said wavelength; and

comparing said absorption at said wavelength with known absorption coefficients for various concentrations of said component to determine the concentration of said component.

16. The method of determining the concentration of a component chemical species in an electromagnetic energy scattering medium which comprises the steps of

exciting a volume of said medium with electromagnetic energy at a predetermined wavelength to cause said medium to generate acoustic energy within said medium whose amplitude is dependent upon the wavelength and intensity of said electromagnetic energy and the absorption coefficient of said component species at said wavelength;

detecting said acoustic energy and generating a signal representative of the amplitude of the acoustic energy;

developing a relationship between the amplitude of said acoustic energy in said scattering medium and the degree of absorption of said electromagnetic energy by said medium and said chemical species;

determining from the intensity of said electromagnetic energy and the amplitude of the acoustic signal the absorption coefficient at said wavelength; and

comparing said absorption coefficient at said wavelength with known absorption coefficients for said component at various concentrations to determine the concentration of said component.

17. The method of claim 16 which includes additionally the step of identifying the material composition which comprises the additional step of comparing said absorption coefficient at said wavelength with known absorption coefficients of various components or various concentrations to determine not only the concentration of said component but also to identify the components.

20. The method of determining the concentration of chemical species in an electromagnetic energy scattering medium which comprises the steps of

irradiating said material with electromagnetic energy to cause said medium to generate acoustic energy within said medium whose amplitude is dependent upon the wavelength and intensity of said electromagnetic energy and the absorption coefficient of aid species at said wavelength;

scanning the wavelength of said electromagnetic energy over a predetermined band of wavelengths;

measuring the intensity of said electromagnetic energy at said scanned wavelengths and generating an intensity signal representative of the intensity at each of said scanned wavelengths;

detecting the acoustic energy at said wavelengths to provide an output signal having an amplitude representative of said acoustic energy at said wavelengths;

developing a relationship between the amplitude of said acoustic energy in said medium and the degree of absorption in said medium for each of said scanned wavelengths;

determining from the electromagnetic energy signal and the acoustic energy signal the absorption coefficient of said species and scattering medium at said wavelengths; and

comparing said absorption coefficients at said wavelengths with known absorption coefficients for various concentrations of said species to determine the concentrations of said species.

21. The method as in claim 20 which includes additionally the step of identifying the material composition which comprises the additional step of comparing said absorption coefficients at said scanned wavelengths with known absorption coefficients of various components or various concentrations to determine not only the concentration of said components but also to identify the components.

26. The apparatus for determining the concentration of species in a biological substance comprising

electromechanical means for exciting a volume of said material with electromagnetic energy to cause a finite volume of said material to generate acoustic energy whose amplitude is dependent upon the intensity and wavelength of said electromagnetic energy and the absorption coefficient of said species;

transducer means for detecting said generated acoustic energy and generating a representative acoustic signal;

processing means for developing a relationship between the amplitude of said acoustic energy in said biological substance and the degree of absorption of said electromagnetic energy by said biological substance; and

processing means for receiving said acoustic signal, making use of the relationship and determining the concentration of said species.

28. The apparatus as in claim 26 or 27 in which means are provided for storing the acoustic amplitude signals and averaging said signals over a plurality of scans before determining the concentration.

29. The apparatus as in claim 26 or 27 including gating means to gate the acoustic signal to detect acoustic signals generated at a selected volume within the sample.

27. Apparatus for determining the concentration of a component in an electromagnetic energy scattering medium which comprises

means for irradiating said material with electromagnetic energy at selected wavelengths to cause said medium to generate acoustic energy within said medium whose amplitude is dependent upon the wavelength and intensity of said electromagnetic energy and the absorption coefficient of said component and scattering medium at said wavelength;

means for scanning the selected wavelengths of said electromagnetic energy over a predetermined band;

means for detecting the acoustic energy at said wavelengths and providing an output signal having an amplitude representative of said acoustic energy at said wavelengths;

means for determining from the acoustic amplitude signal and the intensity of said electromagnetic energy the absorption coefficient of said component and scattering medium at said wavelengths; and

means for comparing said absorption coefficients at said wavelengths with known absorption coefficients for various concentrations of said components to determine the concentration of said components.

30. The apparatus as in claim 27 in which the component is within biological tissue.

31. The apparatus as in claim 27 in which the component is in in-vitro material.