Long-wavelength fluorescent probe compounds for calcium ions and their use in ratiometrically measuring calcium ion concentrations

• US 5,409,835 A
• Filed: 12/30/1992
• Issued: 04/25/1995
• Est. Priority Date: 12/30/1992
• Status: Expired due to Term

• Current Assignee: Joseph R. Lakowicz
• Original Assignee: University of Maryland
• Inventors: Akkaya, Engin U., Lakowicz, Joseph R.
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): Carpenter, Robert
• Application Number:
  US07/998,849
• Time in Patent Office: 846 Days
• Field of Search: 436/63, 436/79, 436/172, 436/800, 544/92, 544/105, 546/121, 546/170, 548/148, 548/149, 548/150, 548/180, 549/23
• US Class Current: 436/79
• CPC Class Codes: C07D 277/64 with only hydrocarbon or substituted hydrocarbon radicals attached in position 2
• CPC Class Codes: G01N 33/52 Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH
• CPC Class Codes: Y10S 436/80 Fluorescent dyes, e.g. rhodamine

Abstract

Fluorescent, calcium-binding probe compounds having visible-light excitation and emission wavelengths, and a method of usage for these probe compounds in making wavelength-ratiometric or intensity-ratiometric measurements of calcium ion concentration in samples. The probe compounds generally relate to a heterocyclic part as bonded to a Ca²⁺ binding unit, namely, BAPTA, via an ethylenically unsaturated group; preferred examples of the heterocyclic group include benzothiazole, naphthothiazole, thiaflavin, indolenine, chloroindolenine, methoxybenzothiazole, and methoxyindolenine.

Claims

1. A fluorescent calcium-binding compound for making wavelength-ratiometric or intensity-ratiometric measurements of calcium ion concentrations having the formula:

##STR4## where n represents an integer of 1, 2 or 3 and R represents thiaflavin or ##STR5## wherein R₂ is selected from the group consisting of methyl, ethyl, sulfopropyl, sulfobutyl, and carboxymethyl, Y is selected from the group consisting of S, (CH₃)₂ C, and O;

W is selected from the group consisting of H, Cl, and OCH₃ ; and

Z is an optional fused aromatic ring.

2. The fluorescent calcium-binding compound of claim 1, wherein R is selected from the group consisting of benzothiazole, napthothiazole, thiaflavin, indolenine, chloroindolenine, methoxybenzothiazole, and methoxyindolenine.

3. A fluorescent calcium-binding compound for making wavelength-ratiometric or intensity-ratiometric measurements of calcium ion concentrations having the formula:

##STR6##

4. A method of making wavelength-ratiometric or intensity-ratiometric measurements of calcium concentration in a sample containing calcium ions using a wavelength of about 400 nm or more, comprising

contacting a sample containing calcium ions with a probe compound of the following formula in sufficient quantity to act as a fluorescent optical indicator independent of concentration of said probe compound:

##STR7## wherein n represents an integer of 1, 2 or 3 and R represents thiaflavin or ##STR8## wherein R₂ is selected from the group consisting of methyl, ethyl, sulfopropyl, sulfobutyl, and carboxymethyl, Y is selected from the group consisting of S, (CH₃)₂ C, and O;

W is selected from the group consisting of H, Cl, and OCH₃ ; and

Z is an optional fused aromatic ring; and

measuring the calcium concentration in the sample by wavelength-ratiometry or intensity-ratiometry using a wavelength of about 400 nm or more.

5. The method of claim 4, wherein R is selected from the group consisting of benzothiazole, napthothiazole, thiaflavin, indolenine, chloroindolenine, methoxybenzothiazole, and methoxyindolenine.

6. The method of claim 4, wherein said sample contains biological tissues.

7. The method of claim 4, wherein said measuring is effected by flow cytometry and is used to measure intracellular calcium concentration.

8. The method of claim 4, wherein said measuring comprises forming two or three-dimensional calcium concentration images in fluorescence microscopy.

9. The method of claim 4, wherein said measuring comprises the following steps:

(1) illuminating said sample with a light source at a first excitation visible wavelength λ

₁ and measuring the resulting fluorescence intensity I₁ radiated from the sample, then

(2) illuminating said sample with a light source at a second excitation visible wavelength λ

₂ and measuring the resulting fluorescence intensity I₂, wherein λ

₁ and λ

₂ are different, and

(3) converting the fluorescence intensities I₁ and I₂ into electrical signals, determining a ratio therefrom, and determining the concentration of calcium in said sample with said ratio.

10. The method of claim 4, wherein said measuring comprises the following steps:

(1) illuminating said sample containing calcium ions with a light source at a excitation visible wavelength λ and

measuring the resulting fluorescence intensity I'"'"' at emission wavelength λ

'"'"',

(2) illuminating said sample containing calcium ions with a light source also at excitation wavelength λ and

measuring the resulting fluorescence intensity I" at emission wavelength λ

", wherein λ

'"'"' and λ

" are different, and

(3) converting the fluorescence intensities I'"'"' at λ

'"'"' and I" at λ

" into electrical signals, determining a ratio therefrom, and determining the concentration of calcium in said sample containing calcium ions with said ratio.

11. The method of claim 9, wherein said excitation visible wavelengths λ

₁ and λ

₂ are each greater than about 400 nm.

12. The method of claim 9, wherein said excitation visible wavelengths λ

₁ and λ

₂ are each selected from a range of about 400 nm to about 670 nm.

13. A method of making wavelength-ratiometric or intensity-ratiometric measurements of calcium concentration in a sample containing calcium ions using a wavelength of about 400 nm or more, comprising

contacting a sample containing calcium ions with a probe compound of the following structure in sufficient quantity to act as a fluorescent indicator independent of concentration of said probe compound:

##STR9## and measuring the calcium concentration in the sample by wavelength-ratiometry or intensity-ratiometry using a wavelength of about 400 nm or more.

14. The method of claim 13, wherein said measuring comprises the following steps:

(1) illuminating said sample with a light source at a first excitation visible wavelength λ

₁ and measuring the resulting fluorescence intensity I₁ radiated from the sample, then

(2) illuminating said sample with a light source at a second excitation visible wavelength λ

₂ and measuring the resulting fluorescence intensity I₂, wherein λ

₁ and λ

₂ are different, and

(3) converting the fluorescence intensities I₁ and I₂ into electrical signals, determining a ratio therefrom, and determining the concentration of calcium in said sample with said ratio.

15. The method of claim 13, wherein said measuring comprises the following steps:

(1) illuminating said sample containing calcium ions with a light source at a excitation visible wavelength λ and

measuring the resulting fluorescence intensity I'"'"' at emission wavelength λ

'"'"',

(2) illuminating said sample containing calcium ions with a light source also at excitation wavelength λ and

measuring the resulting fluorescence intensity I" at emission wavelength λ

", wherein λ

'"'"' and λ

" are different, and

(3) converting the fluorescence intensities I'"'"' at λ

'"'"' and I" at λ

" into electrical signals, determining a ratio therefrom, and determining the concentration of calcium in said sample containing calcium ions with said ratio.

16. The method of claim 14, wherein said excitation visible wavelengths λ

₁ and λ

₂ are each greater than about 400 nm.

17. The method of claim 14, wherein said excitation visible wavelengths λ

₁ and λ

₂ are each selected from a range of about 400 nm to about 670 nm.

18. The method of claim 15, wherein said emission wavelengths λ

'"'"' and λ

" are each greater than 500 nm.