Electrode having a polymer coating with a redox enzyme bound thereto, the polymer coating being formed on the walls of pores extending through a porous membrane
First Claim
1. An electrode comprising a membrane having open pores running through said membrane, the walls of the pores having an electrically conducting polymer coating applied thereto, which polymer coating is in direct electronic contact with a redox enzyme bound thereto;
- said redox enzyme converting to one of an oxidized form and a reduced form upon reacting with a selected substrate, and said electrically conducting polymer participating in direct electron transfer with said redox enzyme to regenerate the other of said oxidized form and reduced form; and
wherein said pores of said membrane remain open following application thereto of said polymer coating.
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Abstract
An electrode is composed of a membrane, provided with open pores running through said membrane, the walls of the pores having an electrically conducting polymer coating, containing a redox enzyme bound thereto. In these type of electrodes a direct electron transfer is possible between the redox enzyme, e.g. glucose oxidase, and the electrically conducting polymer, e.g. polypyrrole. Such an electrode, which can be produced in a simple manner, has extensive application possibilities such as, for example, in a biosensor or in a production installation for the preparation of specific chemicals. As starting materials for the electrodes of the invention, use can be made of marketed porous membrane materials as well as of latex particles. The walls of the pores of porous membrane and the interstices of the latex particles respectively are provided with a thin layer of the electrically conducting polymer which in turn is provided with a redox enzyme suitable for the pursued aim.
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16 Claims
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1. An electrode comprising a membrane having open pores running through said membrane, the walls of the pores having an electrically conducting polymer coating applied thereto, which polymer coating is in direct electronic contact with a redox enzyme bound thereto;
- said redox enzyme converting to one of an oxidized form and a reduced form upon reacting with a selected substrate, and said electrically conducting polymer participating in direct electron transfer with said redox enzyme to regenerate the other of said oxidized form and reduced form; and
wherein said pores of said membrane remain open following application thereto of said polymer coating. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
- said redox enzyme converting to one of an oxidized form and a reduced form upon reacting with a selected substrate, and said electrically conducting polymer participating in direct electron transfer with said redox enzyme to regenerate the other of said oxidized form and reduced form; and
Specification