Method for stabilizing glucose 6-phosphate dehydrogenase with hydroxylamines, aldehyde scavengers, dimethylthiocarbamoyl chloride or 2-(2-aminoethylamino) ethanol
First Claim
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1. A method for preparing an assay reagent containing stabilized glucose 6-phosphate dehydrogenase comprising adding to glucose 6-phosphate dehydrogenase a stabilizing amount of at least one stabilizer selected from the group consisting of dimethylthiocarbamoyl chloride, 2-(2-aminoethylamino) ethanol, a hydroxylamine which is a compound represented by formula (I):
- ##STR13## wherein R1, R2 and R3 may be either the same or different and each represents a hydrogen atom, a substituted or unsubstituted lower alkyl group, a cycloalkyl group, a lower alkenyl group, a substituted or unsubstituted aralkyl group, a substituted or unsubstituted aryl group, a lower alkanoyl group, a substituted or unsubstituted aroyl group, a cinnamyl group or a cinnamoyl group, or R2 and R3 together form a nitrogen-containing heterocyclic group by interposing a nitrogen atom, an aldehyde scavenger which is a compound represented by formula (II) ##STR14## wherein R4 and R5 may be either the same or different and each represents a hydrogen atom, a substituted or unsubstituted lower alkyl group, a cycloalkyl group, a lower alkenyl group, a substituted or unsubstituted aralkyl group, a substituted or unsubstituted aryl group, a substituted or unsubstituted heterocyclic group, a lower alkanoyl group, a substituted or unsubstituted aroyl group, a pyridylcarbonyl group, a lower alkoxycarbonyl group or a substituted or unsubstituted amino group, X represents an oxygen atom or a sulfur atom, and n is an integer of 0 to 2, and a salt thereof to produce said assay reagent.
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Abstract
Glucose 6-phosphate dehydrogenase is stabilized in a clinical assay reagent with at least one stabilizer selected from among hydroxylamines, aldehyde scavengers dimethylthiocarbamoyl chloride, 2-(2-aminoethylamine) ethanol, and their salts. The reagent may be in liquid or lyophilized form.
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4 Claims
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1. A method for preparing an assay reagent containing stabilized glucose 6-phosphate dehydrogenase comprising adding to glucose 6-phosphate dehydrogenase a stabilizing amount of at least one stabilizer selected from the group consisting of dimethylthiocarbamoyl chloride, 2-(2-aminoethylamino) ethanol, a hydroxylamine which is a compound represented by formula (I):
- ##STR13## wherein R1, R2 and R3 may be either the same or different and each represents a hydrogen atom, a substituted or unsubstituted lower alkyl group, a cycloalkyl group, a lower alkenyl group, a substituted or unsubstituted aralkyl group, a substituted or unsubstituted aryl group, a lower alkanoyl group, a substituted or unsubstituted aroyl group, a cinnamyl group or a cinnamoyl group, or R2 and R3 together form a nitrogen-containing heterocyclic group by interposing a nitrogen atom, an aldehyde scavenger which is a compound represented by formula (II) ##STR14## wherein R4 and R5 may be either the same or different and each represents a hydrogen atom, a substituted or unsubstituted lower alkyl group, a cycloalkyl group, a lower alkenyl group, a substituted or unsubstituted aralkyl group, a substituted or unsubstituted aryl group, a substituted or unsubstituted heterocyclic group, a lower alkanoyl group, a substituted or unsubstituted aroyl group, a pyridylcarbonyl group, a lower alkoxycarbonyl group or a substituted or unsubstituted amino group, X represents an oxygen atom or a sulfur atom, and n is an integer of 0 to 2, and a salt thereof to produce said assay reagent.
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Current AssigneeKyowa Medex Co., Ltd. (Resonac Holdings Corp.)
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Original AssigneeKyowa Medex Co., Ltd. (Resonac Holdings Corp.)
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InventorsSakakibara, Minako, Aoyama, Norihito
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Primary Examiner(s)Naff, David M.
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Assistant Examiner(s)MELLER, MICHAEL V
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Application NumberUS08/294,586Time in Patent Office294 DaysField of Search435/190, 435/188, 435/14, 435/26US Class Current435/188CPC Class CodesA61K 38/00 Medicinal preparations cont...C12N 9/0006 acting on CH-OH groups as d...C12N 9/96 Stabilising an enzyme by fo...
