Methods for reducing level of interferants in biosensor systems and solutions used in these methods
First Claim
1. A method for measuring methanol concentration wherein a liquid sample is brought into contact with an enzyme to form a polarographically measurable substance and wherein formaldehyde is present as an undesirable interferant in said method, the improvement comprising contacting said formaldehyde with a polyethyleneimine formaldehyde scavenger compound.
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Accused Products
Abstract
Improved methods and compositions are provided to inhibit interference from formaldehyde in polarographic analytic methods. In the enzymatic oxidation of methanol to hydrogen peroxide and formaldehyde, formaldehyde serves as an interfering agent in systems where the oxygen consumed or the hydrogen peroxide produced is polarographically measured. To inhibit this interference, an amine-containing formaldehyde scavenger is added to the test system. Preferably, the scavenger comprises a polyalkyleneimine such as polyethyleneimine having a molecular weight of between about 200-1,000,000 amu. The invention is particularly well-suited for use in polarographic analytical apparatus wherein laminated membrane type enzyme electrodes are used to perform amperometric measurement with alcohol oxidase enzyme used as the oxidoreductase enzyme. Determination of methanol concentration in liquid samples can be used to assess the amount of L-Asp-L-Phe-methyl ester (aspartame-TM) present in diet soft drink samples that have been hydrolyzed with the enzyme Bovine alpha-chymotrypsin to yield L-Asp-L-phe and methanol.
11 Citations
15 Claims
- 1. A method for measuring methanol concentration wherein a liquid sample is brought into contact with an enzyme to form a polarographically measurable substance and wherein formaldehyde is present as an undesirable interferant in said method, the improvement comprising contacting said formaldehyde with a polyethyleneimine formaldehyde scavenger compound.
- 6. A method for determining methanol concentration level in a liquid sample with an enzyme containing laminated membrane assembly wherein an oxidoreductase enzyme is disposed within said laminated membrane and wherein said methanol is placed in contact with said enzyme to form hydrogen peroxide and formaldehyde, the improvement comprising contacting said formaldehyde with a polyethyleneimine polymer having a molecular weight of between about 200 and 1,000,000.
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15. A method for determining methanol concentration of a methanol containing analyte sample using a polarographic cell having an enzyme containing laminated membrane in close proximity to a working electrode of said cell, wherein said laminated membrane comprises an outer barrier membrane and an inner membrane adjacent said working electrode and wherein said enzyme comprises alcohol oxidase enzyme disposed between said inner and outer membranes, wherein the method comprises the steps of:
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(a) contacting the outer membrane of the cell with an analyte sample; (b) allowing said methanol to pass through said outer barrier layer while inhibiting passage of higher molecular weight species having molecular weights of greater than 1,000,000 through said outer layer; (c) oxidizing said methanol with said alcohol oxidase to form hydrogen peroxide and formaldehyde; (d) reacting said formaldehyde formed in said step (c) with an polyethyleneimine formaldehyde scavenger having a molecular weight of between about 200-1,000,000; (e) allowing said hydrogen peroxide to pass through said inner membrane to gain access to said working electrode while inhibiting passage of compounds having a molecular weight of 200 or greater therethrough, and (f) polarographically measuring hydrogen peroxide present at said working electrode.
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Specification