Enzyme-electrode sensor

• US 5,437,973 A
• Filed: 05/07/1993
• Issued: 08/01/1995
• Est. Priority Date: 09/16/1985
• Status: Expired due to Fees

• Current Assignee: The Victoria University of Manchester
• Original Assignee: The Victoria University of Manchester
• Inventors: Vadgama, Pankaj M., Mullen, William, Churchouse, Stephen
• Primary Examiner(s): Snay, Jeffrey R.
• Application Number:
  US08/058,037
• Time in Patent Office: 816 Days
• Field of Search: 435/4, 435/11, 435/14, 435/288, 435/291, 435/817, 422/80, 422/82.01, 436/63, 436/68, 436/151, 204/153, 204/12, 204/403, 204/409, 204/415
• US Class Current: 205/778
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A sensor of the enzyme-electrode type containing a layer of porous material of restricted permeability between the enzyme and a sample to be analysed. The porous material has a percentage porosity not greater than 5% and preferably in the range 0.005% to 0.5%.

Claims

1. An enzyme-electrode sensor for determining an analyte, which analyte is converted by an enzyme into a species detectable by the sensor, said sensor comprising:

an electrode; and

a membrane, superimposed on said electrode in physical contact therewith, said membrane being permeable to liquids and solutes, and wherein said membrane further comprises a first layer containing at least one enzyme, and a second layer, disposed on that side of the first layer which is further from said electrode, which second layer includes an area of porous material of restricted permeability through which the analyte can pass and has a porosity within a range of 0.001 to 0.5 percent and a mean pore diameter that does not exceed 0.01 microns.

2. An enzyme-electrode sensor according to claim 1, wherein said membrane comprises in addition an inner layer, disposed between said first layer and said electrode.

3. An enzyme-electrode sensor for determining an analyte, which analyte is converted by an enzyme into a species detectable by the sensor, said sensor comprising:

an electrode; and

a membrane permeable to liquids and solutes, positioned in physical contact with the electrode, said membrane comprising at least;

a) a first layer,

b) a second layer overlying and contacting said first layer and disposed on a side of said first layer farther from said electrode, and

c) an inner layer underlying said first layer,

wherein said first layer contains at least one enzyme,

wherein said second layer has a porosity lying within the range of 0.001 to 0.5 percent and wherein a mean pore diameter does not exceed 0.01 microns and is selected from the group consisting of polycarbonates, polyurethanes and modified cellulose; and

wherein said inner layer is disposed between said first layer and said electrode and comprises a porous material of a type which restricts or prevents the passage of electroactive interfering compounds.

4. An enzyme-electrode sensor according to claim 3 wherein said inner layer comprises a polymeric material selected from the group consisting of polymethyl methacrylate, polyurethane and cellulose acetate.

5. A method for determining an analyte in a specimen, which comprises the steps of:

contacting a specimen to be determined with a membrane which is permeable to liquids and solutes and comprises at least two layers, a first of which layers comprises at least one enzyme, which convert the analyte into a species detectable by an electrode sensor, and a second of which layers, disposed between said first layer and the specimen to be analyzed, contains an area through which analyte can pass, formed from a porous material of restricted permeability having a porosity lying within the range 0.001 to 0.5 percent and a mean pore diameter no more than 0.01 microns;

detecting said species by said sensor; and

measuring a response of said electrode sensor to said species to determine the concentration of said analyte in the specimen.

6. An enzyme-electrode sensor for determining an analyte, which analyte is converted by an enzyme into a species detectable by the sensor, said sensor comprising:

an electrode; and

a membrane permeable to liquids and solutes, positioned in physical contact with the electrode, said membrane comprising at least;

a) a first layer,

b) a second layer overlying and contacting said first layer and disposed on a side of said first layer further from said electrode, and

c) an inner layer underlying said first layer,

wherein said second layer has a porosity such that it has a restricted permeability, and is formed from a material selected from the group consisting of polycarbonates, polyurethanes and modified cellulose, and wherein said second layer has a porosity in the range of from 0.001 to 0.05 percent and a mean pore diameter no larger than 0.01 microns; and

wherein said inner layer is disposed between said first layer and said electrode and comprises a porous material of a type which restricts or prevents the passage of electroactive interfering compounds and wherein said first layer includes at least one enzyme immobilized beneath the second layer.

7. An enzyme-electrode sensor according to claim 6, wherein the enzyme is immobilised within a layer between the second layer and the inner layer and also within the pores of the inner layer.