Reduced and controlled surface binding of biologically active molecules

• US 5,441,739 A
• Filed: 03/11/1993
• Issued: 08/15/1995
• Est. Priority Date: 06/22/1990
• Status: Expired due to Term

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Gelman, Andrew E., Kossovsky, Nir, Sponsler, Edward E.
• Primary Examiner(s): Page, Thurman K.
• Assistant Examiner(s): Spear, James M.
• Application Number:
  US08/029,896
• Time in Patent Office: 887 Days
• Field of Search: 424/461, 424/479, 424/480, 424/490, 424/493, 424/494, 424/400
• US Class Current: 424/400
• CPC Class Codes: A61K 47/26 Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
• CPC Class Codes: A61K 47/6923 the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb
• CPC Class Codes: A61K 47/6929 the form being a nanoparticle, e.g. an immuno-nanoparticle
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: A61K 9/0019 Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner non-active ingredients are additionally classified in A61K47/00
• CPC Class Codes: A61K 9/0026 Blood substitute; Oxygen transporting formulations; Plasma extender
• CPC Class Codes: A61K 9/127 Liposomes
• CPC Class Codes: A61K 9/5115 Inorganic compounds
• CPC Class Codes: A61K 9/5161 Polysaccharides, e.g. alginate, chitosan, cellulose derivatives; Cyclodextrin
• CPC Class Codes: A61L 31/10 Macromolecular materials
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 11/14 Enzymes or microbial cells immobilised on or in an inorganic carrier
• CPC Class Codes: C12N 15/87 Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
• CPC Class Codes: C12N 2710/16222 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2740/16022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

Articles of manufacture which are adapted for use in contact with one or more biologically active agents are coated with a glassy carbohydrate film. The glassy film provides a reduced surface energy coating which exhibits a reduced degree of binding with biologically active agents. Methods for applying the glassy carbohydrate film are disclosed wherein the glassy film is adsorbed directly onto the article surface. The coated articles are for use both in vitro and in vivo where contact with biologically active agents is expected.

Claims

1. An article of manufacture which is adapted for use in contact with one or more biologically active agents, said article of manufacture being selected from the group consisting of containers, implant devices, electrodes, pumps, syringes, needles, conduits, applicators, bio/opto-electric devices, tubing, said article of manufacture comprising a surface, said surface comprising a ceramic, metal or polymer having a high surface energy wherein said surface binds to said biologically active agent or agents with a certain binding energy, the improvement comprising adsorbing directly to said surface a glassy carbohydrate film, said film consisting of an oligosaccharide selected from the group of sugars consisting of cellobiose, trehalose, isomaltose, and nystose or an allosteric effector, selected from the group consisting of pyridoxal-5-phosphate and 2,3-phosphoglycerate said glassy film having a surface energy which is below the surface energy of said surface to thereby provide a reduction in the binding energy between said surface and said biologically active agent or agents.

2. An improved article of manufacture according to claim 1 wherein said article comprises a conduit for materials which comprise a biologically active agent.

3. An improved article of manufacture according to claim 1 wherein said oligosaccharide comprises a sugar selected from the group of basic sugars consisting of cellobiose, trehalose, isomaltose, and nystose.

4. An improved article of manufacture according to claim 1 wherein said glassy film comprises an allosteric effector selected from the group consisting of pyridoxal-5-phosphate and 2,3-phosphoglycerate.

5. An improved article of manufacture according to claim 3 wherein said glassy film comprises an allosteric effector.

6. An improved article of manufacture according to claim 1 wherein said ceramic is glass.

7. An improved article of manufacture according to claim 1 wherein said article is a container for materials which comprise biologically active agents.

8. An improved article of manufacture according to claim 6 wherein said oligosaccharide is cellobiose.